The radiation protection role of heparin-SOD conjugate in irradiated mice

  • Chuanqing Zhong Shandong Jianzhu University; School of Municipal and Environmental Engineering
  • Xiaojing Huang Henan Provincial People’s Hospital; Department of Pharmacy
  • Shu Zhang Shandong University; School of Pharmaceutical Science; Institute of Biochemical and Biotechnological Drugs
  • Yanna Cheng Shandong University; School of Pharmaceutical Science; Institute of Biochemical and Biotechnological Drugs
  • Guangxiang Cao Shandong Medicinal Biotechnology Centre
  • Fengshan Wang Shandong University; School of Pharmaceutical Science; Institute of Biochemical and Biotechnological Drugs
Keywords: Heparin-superoxide dismutase conjugate, Radiation-induced damage, Oxidative stress injury

Abstract

Heparin-SOD conjugate (Hep-SOD) was prepared by modifying Cu,Zn-SOD with heparin. An acute radiation-induced mouse injury model was constructed to study the radiation protection effects of Hep-SOD conjugate. Fifty-six mice were randomly divided into seven groups: (I) normal control group; (II) irradiated control group; (III) positive control group (amifostine group, 300 mg/kg); (IV) SOD group (35000 U/kg); (V) high dosage of Hep-SOD group (70000 U/kg); (VI) medium dosage of Hep-SOD group (35000 U/kg); (VII) low dosage of Hep-SOD group (17500 U/kg). Drugs were intraperitoneally injected into each mouse 1 h before radiation except for the normal control group. All the irradiated groups were irradiated with 6 Gy. Organ indices, haematopoietic function indices, peripheral blood cells, liver function test, oxidative stress state and pathological observation were detected to study the effects of Hep-SOD on irradiated mice. Results showed that bone marrow suppression of irradiated mice could be reduced when treated by Hep-SOD before radiation. Oxidative stress detection and pathological observation of the liver and intestine showed that the damage caused by radiation was relieved when mice were treated with Hep-SOD before radiation. This study shows a new direction to prevent organisms from the damage caused by radiation.

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Published
2017-01-01
How to Cite
Zhong, C., Huang, X., Zhang, S., Cheng, Y., Cao, G., & Wang, F. (2017). The radiation protection role of heparin-SOD conjugate in irradiated mice. Brazilian Journal of Pharmaceutical Sciences (Impresso), 53(4), e17081. https://doi.org/10.1590/s2175-97902017000417081
Section
Articles