Antidiabetic activity evaluation of Onobrychis species on alloxaninduced diabetic mice

  • Hanefi Ozbek İstanbul Medipol University, Faculty of Medicine, Department of Pharmacology
  • Ozlem Bahadir Acikara Ankara University, Faculty of Pharmacy, Department of Pharmacognosy
  • Burcin Ergene Oz Ankara University, Faculty of Pharmacy, Department of Pharmacognosy
  • Serkan Ozbilgin Ankara University, Faculty of Pharmacy, Department of Pharmacognosy
  • Neriman Ipek Kirmizi İstanbul Medipol University, IMU Vocational School, Department of Occupational Health and Safety
  • Bade Cevriye Ozrenk İstanbul Medipol University, IMU Vocational School, Department of Occupational Health and Safety
  • Mehmet Tekin Trakya University, Faculty of Pharmacy, Department of Pharmaceutical Botany
  • Gulcin Saltan Ankara University, Faculty of Pharmacy, Department of Pharmacognosy
Keywords: Onobrychis, Antidiabetic activity, Alloxan, Rutin, Isoquercetin, Phytochemical analysis

Abstract

In the current study, four Onobrychis species, O. albiflora Hub.-Mor., O. argyrea Boiss. subsp. argyrea Boiss., O. galegifolia Boiss., and O. tournefortii (Willd.) Desv. were collected from Anatolia to be evaluated for their antidiabetic activities. Methanol water extracts of the aerial parts were used for experiments. An alloxan-induced diabetic mice test model was used. Phytochemical analysis of the tested extracts was investigated using the HPLC method. The highest activity was observed with treatment of O. albiflora aerial part extract. Significant decrements were detected in the blood glucose levels as follows: 180.83±47.48 and 252.83±50.47mg/dL at 100 mg/kg and 200 mg/kg doses of O. albiflora, respectively, when compared to the isotonic saline solution control group, eliciting a blood glucose level of 494.20±27.32. Among the tested standard compounds, rutin and isoquercetin were detected in the examined species. The highest amount of rutin (1.1981±0.0017%) and isoquercetin (0.7318±0.0197%) were found in O. albiflora and O. argyrea subsp. argyrea, respectively. Antidiabetic activities of the tested Onobrychis species seem to indicate a possible correlation with their rutin and isoquercetin contents. Therefore, rutin and isoquercetin may be the antidiabetic compounds that contribute to the antidiabetic activity of the tested Onobrychis species.

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Published
2019-08-07
How to Cite
Ozbek, H., Acikara, O., Oz, B., Ozbilgin, S., Kirmizi, N., Ozrenk, B., Tekin, M., & Saltan, G. (2019). Antidiabetic activity evaluation of Onobrychis species on alloxaninduced diabetic mice. Brazilian Journal of Pharmaceutical Sciences, 55, e18157. https://doi.org/10.1590/s2175-97902019000118157
Section
Articles