Assessment of anti-diabetic activity of a novel hydrazine-thiazole derivative: in vitro and in vivo method

  • Marina Ferrara de Resende Federal University of Minas Gerais, Pharmacy Faculty, Department of Foods
  • Cleudiomar Inácio Lino Federal University of Minas Gerais, Pharmacy Faculty, Department of Pharmaceutical Products
  • Elaine Maria de Souza-Fagundes Federal University of Minas Gerais, Department of Physiology and Biophysics
  • João Vitor Paes Rettore Federal University of Juiz de Fora, Institute of Biological Science, Department of Biology
  • Renata Barbosa de Oliveira Federal University of Minas Gerais, Pharmacy Faculty, Department of Pharmaceutical Products
  • Renata Adriana Labanca Federal University of Minas Gerais, Pharmacy Faculty, Department of Foods
Keywords: Oxidative stress, Diabetes mellitus, Curcumin, Thiazole derivatives, Dietary supplement

Abstract

Diabetes mellitus is a chronic disease resulting in oxidative stress that promotes tissue damage. The appearance of this disease is highly related to lifestyle and food of the population, being of great interest to search for a dietary supplement that can also act by reducing oxidative alterations. Based on the broad range of biological activity of thiazole derivatives, this work aimed to evaluate the in vitro antioxidant activity of a novel hydrazine-thiazole derivative and studies in vivo. In in vivo experiments, the liver extracts of healthy and diabetic Wistar rats were used, with analysis to determine the enzymatic activity of SOD, CAT, GPx, and GR, and determination of lipid peroxidation. Finally, in the blood of these animals, biochemical parameters were evaluated. Statistical evidence of changes caused in liver enzymes and liquid peroxidation was not detected; however, these parameters were also not changed between control groups with and without diabetes. On the other hand, concerning biochemical parameters, significant differences were detected in uric acid, alkaline phosphatase, ALT, and urea, indicating a possible antioxidant protective role of such substances in the liver and kidney of diabetic animals that could be acting by means other than that commonly reported in the literature.

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Published
2019-08-08
How to Cite
Resende, M., Lino, C., Souza-Fagundes, E., Rettore, J. V., Oliveira, R., & Labanca, R. (2019). Assessment of anti-diabetic activity of a novel hydrazine-thiazole derivative: in vitro and in vivo method. Brazilian Journal of Pharmaceutical Sciences, 55, e18218. https://doi.org/10.1590/s2175-97902019000118218
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Articles