Toxicity assessment of the alcoholic leaves extract of Reinwardtia indica

  • Prabhat Upadhyay Banaras Hindu University, Institute of Medical Sciences, Department of Pharmacology
  • Rashmi Shukla Banaras Hindu University, Institute of Medical Sciences, Department of Medicinal Chemistry
  • Kavindra Nath Tiwari MMV Banaras Hindu University, Department of Botany
  • G P Dubey Banaras Hindu University, Institute of Medical Sciences
  • Sunil Kumar Mishra Banaras Hindu University, Indian Institute of Technology, Department of Pharmaceutical, Engineering and Technology
Keywords: Reinwardtia indica, Biochemical test, Hematological test, Histopathology

Abstract

The present study aimed to evaluate the safety of the alcoholic leaves extract of Reinwardtia indica in Charles foster rats through an acute and sub-acute oral administration.For assessment of acute oral toxicity test, ratswere orally treated with single dose of the alcoholic leaves extract of Reinwardtia indica at the doses of 50, 250, 500, 1000 2000 and 5000 mg/kg. In sub-acute toxicity study, using the OECD guidelines no. 407, the extract was administered at the doses of 50, 250, 500, 1000, 2000 mg/kg/day for 28 consecutive days and at the dose of 2000 mg/kg satellite group also used for 6 weeks.In acute toxicity above mentioned doses neither showed mortality nor exterior signs of toxicity. In sub-acute, study no significant changes found in haematological and biochemical level ofthe treated rat after 14 days and 28 days in comparison to control. The histopathology of rat brain, kidney, liver, and heart also showed the no cellular changes after extract treated rat.The alcoholic leaves extract of Reinwardtia indica was found non-toxic in single drug dose administration up to 5000 mg/kg (acute study) and in sub-acute administration up to 2000 mg/kg.

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Published
2019-08-08
How to Cite
Upadhyay, P., Shukla, R., Tiwari, K., Dubey, G., & Mishra, S. (2019). Toxicity assessment of the alcoholic leaves extract of Reinwardtia indica. Brazilian Journal of Pharmaceutical Sciences, 55, e18224. https://doi.org/10.1590/s2175-97902019000118224
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Articles