Curcumin combining with si-MALAT1 inhibits the invasion and migration of colon cancer SW480 cells

  • Dai Wei Gangzhou People’s Hospital
  • LI Shi Yun Gangzhou People’s Hospital
  • Xiao Dejun Gangzhou People’s Hospital
  • Liu Cong Gangzhou People’s Hospital
  • Jin-Hua He Central Hospital of Panyu District
  • Lin Yan Gangzhou People’s Hospital https://orcid.org/0000-0003-4956-861X
Keywords: Curcumin/ pharmacology, LncRNA-MALAT1, RNA, Small Interfering/ drug effects, Colon cancer, Invasion, Migration, Colonic Neoplasms/ prevention & control, Colonic Neoplasms/ drug therapy, Neoplasm Invasiveness/ prevention & control, Cell Migration Inhibition/ drug effects

Abstract

To study the effect of small interfering RNA targeting metastasis-associated lung adenocarcinoma transcript1 (si-MALAT1) combining with curcumin on the invasion and migration abilities of human colon cancer SW480 cells, and to explore the involved molecular mechanism. The recombinant lentiviral vector expressing si-MALAT1 was constructed, and its titer was determined by gradient dilution method. The colon cancer SW480 cells with stable expression of si-MALAT1 was established, followed by treatment with curcumin at different concentrations. The effect of curcumin or si-MALAT1 alone and the combination of the two on the cell activity was detected by MTT assay. The cell invasion and migration abilities were detected by transwell and scratch-wound assay. The relative expression level of MALAT1 was detected by RT-qPCR. The protein expression was determined by Western blot analysis. The IC50 of curcumin alone was 77.69 µmol/L, which was 51.17 mol/Lwhen combined with curcumin and random sequence. The IC50 of curcumin was 30.02 µmol/L when combined with si-MALAT1. The increased susceptibility multiples was 2.58. The wound healing rates were 30.9% and 67.5% after treatment with si-MALAT1 combined with curcumin for 24 hrs and 48 hrs, respectively. The numbers of invasion cells were 200±12, 162±13, 66±8, 53±4 and 16±3 after treatment with si-MALAT1 combined with curcumin for 48 hrs. The relative expression level of lncRNA-MALAT1 in the curcumin group was 68%, and the relative expression level of lncRNA-MALAT1 in si-MALAT1group was 56%, and that for the combination treatment group was about 21%. The protein expression levels of β- catenin, c-myc and cyclinD1 were significantly down-regulated upon treatment with certain concentration of si-MALAT1 alone or combined with curcumin.si-MALAT1 could significantly inhibit the invasion and migration of SW480 cells by enhancing the sensitivity of SW480 cells to curcumin. The mechanism involved mignt be related to the down-regulation of β-catenin, c-myc and cyclinD1 proteins.

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Published
2019-11-28
How to Cite
Wei, D., Yun, L., Dejun, X., Cong, L., He, J.-H., & Yan, L. (2019). Curcumin combining with si-MALAT1 inhibits the invasion and migration of colon cancer SW480 cells. Brazilian Journal of Pharmaceutical Sciences, 55, e18276. https://doi.org/10.1590/s2175-97902019000118276
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Articles