Amphotericin-B and vancomycin-loaded chitosan nanofiber for antifungal and antibacterial application

  • Soroush Karimi Kermanshah University of Medical Sciences, Nano Drug Delivery research center
  • Pouran Moradipour Kermanshah University of Medical Sciences, Nano Drug Delivery research center
  • Abbas Hemati Azandaryani Kermanshah University of Medical Sciences, Nano Drug Delivery research center
  • Elham Arkan Kermanshah University of Medical Sciences, Nano Drug Delivery research center https://orcid.org/0000-0001-5518-2145
Keywords: Mucoadhesive, Vancomycin, Amphotericin-B, Electrospinning, Aphthous, Chitosan

Abstract

In the present study, a mucoadhesive non-woven fiber mat (d= 116 nm) was fabricated by the electrospinning method using chitosan (80% Wt), polyethylene oxide (10% Wt), cysteine (4% Wt) and drugs (6% Wt), respectively. In addition, a comparative study was conducted to define effect of drugs and mucoadhesive agent on the nanofiber formation. FTIR, SEM, DSC and DMA were used to investigate the chemical and physical properties of the nanofibers. In vitro release of the drugs was assessed over a 48-hour period by the total immersion method. Release data were fitted to kinetic models, including the zero-order, first-order, Higuchi matrix, and Hixson–Crowell. Zone inhibition investigations were used to describe the inhibition content of vancomycin and amphotericin B loaded in the mats. The SEM images displayed a slight decrease in the fiber diameter with adding drugs and mucoadhesive agents. FTIR spectra confirmed that any undesirable reaction between VAN–AMB and CS-PEO was not observed. DSC test recognized the uniform distribution of drugs in the polymeric bead of the fiber without any crystal form. The elasticity modulus of the nanofiber was in an acceptable range for oral mucosa (approximately 5 Mpa). The results indicated that biodegradable mucoadhesive nanofibrous membranes released high concentrations of VAN in the first 24 hours, but the AMB release was affected in more controlled phenomena.

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Published
2019-11-29
How to Cite
Karimi, S., Moradipour, P., Azandaryani, A., & Arkan, E. (2019). Amphotericin-B and vancomycin-loaded chitosan nanofiber for antifungal and antibacterial application. Brazilian Journal of Pharmaceutical Sciences, 55, e17115. https://doi.org/10.1590/s2175-97902019000117115
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Articles