Essential oil of the leaves of Eugenia sulcata preserve myocardial contractility and does not present immunotoxicity

  • Karolina Torres Santos Federal University of Pampa
  • Fabricio Ocampo da Luz e Silva Federal University of Pampa
  • Leandro Alves Schneider Federal University of Pampa
  • Carlos Eduardo Rangel dos Santos Federal University of Pampa
  • Sue Elle Berro da Silva Federal University of Pampa
  • Maquelen Blanco Fernandes Federal University of Pampa
  • Leandro Rocha Federal University Fluminense
  • Luís Flávio Souza de Oliveira Federal University of Pampa
  • Michel Mansur Machado Federal University of Pampa
  • Cleci Menezes Moreira Federal University of Pampa https://orcid.org/0000-0002-8080-6965
Keywords: Myrtaceae/adverse effects, Angiotensin-Converting Enzyme Inhibitors/immunology

Abstract

The essential oil of the leaves of Eugenia sulcata, in the Myrtaceae family, has a demonstrated antihypertensive effect, but its effects on heart muscle and its toxicity have not yet been elucidated. Little chemical or biological data are available for E. sulcata, whether emphasizing the beneficial effects or the pharmacological security of this species. This study aims to evaluate myocardial contractility and to analyze angiotensin converting enzyme (ACE) and myosin ATPase activities associated with use of this essential oil. In addition, we evaluated the immunotoxicity of E. sulcata essential oil. Wistar Kyoto (WKY) and spontaneously hypertensive rats (SHR) were treated daily for 30 days (10 mg/kg of oil) to evaluate the isometric force of the papillary muscle, ACE measured by fluorimetry, and myosin ATPase activities by inorganic phosphate. Lymphocyte cultures were used to evaluate cytotoxicity, DNA damage, and mutagenicity of the essential oil. The results demonstrate that the treatment did not change the cardiac contraction force and did not alter the functioning of the sarcoplasmic reticulum, extrusion of the membrane calcium, or modify the membrane calcium channels or β-adrenergic receptor activity. Tetanic contractions were potentiated in the SHR animals. Myosin ATPase activity was also increased in the SHR animals. Cardiac ACE activity was reduced in both animal strains, and the serum ACE was reduced only in the SHR animals. The essential oil did not cause cytotoxicity or mutagenicity and presented low DNA damage. Our results demonstrated that the essential oil does not change myocardial contractility and does not present relevant immunotoxicity.

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Published
2019-12-02
How to Cite
Santos, K., Silva, F., Schneider, L., Santos, C. E., Silva, S., Fernandes, M., Rocha, L., Oliveira, L. F., Machado, M., & Moreira, C. (2019). Essential oil of the leaves of Eugenia sulcata preserve myocardial contractility and does not present immunotoxicity. Brazilian Journal of Pharmaceutical Sciences, 55, e17742. https://doi.org/10.1590/s2175-97902019000117742
Section
Articles