Vasorelaxant activity and acute toxicity of the ethanolic extract of Zanthoxylum rhoifolium Lam leaves

  • Joliane Carvalho Silva Federal University of Piauí
  • Pedro Simão da Silva Azevedo Federal University of Piauí
  • Fabiana de Moura Souza Federal University of Piauí
  • Kalynca Kayla Viana Aragão Federal University of Piauí
  • Carla Kelly Barroso Sabino Federal University of Piauí
  • Gabriela Dantas Carvalho Federal University of Piauí
  • Ilmara Cecília Pinheiro Morais Federal University of Piauí
  • Marcelo Bezerra Mendes Federal University of Piauí
  • Mariana Helena Chaves Federal University of Piauí
  • Aldeídia Pereira de Oliveira
Keywords: Mesenteric arteries/ drug effects, Vasorelaxant, Zanthoxylum rhoifolium/ toxicity, Rats, Wistar, Blood Vessels/ drug effects


The study evaluated the vasorelaxant effect induced by the ethanolic extract of the leaves of Zanthoxylum rhoifolium Lam (EEtOH-Zr/leaves). Wistar rats were treated with the leaf extract containing a single dose of 2,000 mg / kg, v.o. After 14 days, the animals were anesthetized for blood collection and subsequent analysis of the biochemical parameters; they were then euthanized (sodium pentobarbital-100 mg/kg, i.p.) for the removal and morphological analysis of the heart, lung, liver and kidney. The vasorelaxation activity the and vascular reactivity of EEtOH-Zr/leaves were evaluated on artery mesenteric rings isolated from rats. The extract showed no signs of toxicity and no significant difference in the values of the biochemical parameters between the control group and the group of treated animals. In the evaluation of pharmacological activity in the smooth muscle, the EEtOH-Zr/leaves caused vasorelaxant effect on the tonic contraction induced by phenylephrine in mesenteric artery preparations in the presence (pD2=2.17±0.05 µg/mL; Emax=99.8±5.2%) and absence (pD2=2.14±0.05 µg/mL; Emax=95.3±6.4%) of the vascular endothelium. Oral administration of EEtOH-Zr/leaves reduced the contraction induced by the cumulative addition of PHE. It is concluded that the EEtOH-Zr/leaves promote vasorelaxation and reduce vascular reactivity of adrenergic alpha-1 agonist in the mesenteric artery. The results did not show toxic effects of the extract.


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How to Cite
Silva, J., Azevedo, P. S., Souza, F., Aragão, K., Sabino, C., Carvalho, G., Morais, I., Mendes, M., Chaves, M., & Oliveira, A. (2019). Vasorelaxant activity and acute toxicity of the ethanolic extract of Zanthoxylum rhoifolium Lam leaves. Brazilian Journal of Pharmaceutical Sciences, 55, e17754.