Preparation and evaluation of 2-methoxyestradiol-loaded pH-sensitive liposomes

  • Suna He Henan University of Science and Technology, Medical College https://orcid.org/0000-0001-7529-809X
  • Bowen Wang Henan University of Science and Technology, Medical College
  • Runfang Zhang Henan University of Science and Technology, Medical College
  • Huanhuan Zhou Henan University of Science and Technology, Medical College
  • Qian Yang Henan University of Science and Technology, Medical College
Keywords: 2-Methoxyestradiol, pH sensitive liposomes, In vitro release, Stability

Abstract

The development and clinical application of 2-methoxyestradiol (2-ME) as a new type of antitumor drug are limited due to its poor solubility, rapid metabolism in vivo, and large oral dosage. 2-ME-loaded pHsensitive liposomes (2-ME-PSLs) was prepared containing the lipids, Lipoid E-80 (E-80), cholesteryl hemisuccinate (CHEMS), and cholesterol (CHOL) via thin-film ultrasonic dispersion. First, preparation conditions of 2-ME-PSLs were optimized by orthogonal test. Then 2-ME-PSL was characterized, and the release behavior and stability of 2-ME-PSL in vitro were evaluated. The optimal preparation conditions for 2-ME-PSLs were as follows: 2-ME : E-80+CHEMS 1:15; CHOL : E-80+CHEMS 1:5; ultrasonication time 20 minutes. The mean particle size, PDI, zeta potential, and entrapment efficiency (EE) of 2-MEPSLs were 116 ± 9 nm, 0.161 ± 0.025, −22.4 ± 1.7 mV, and 98.6 ± 0.5%, respectively. As viewed under a transmission electron microscope, 2-ME-PSLs were well dispersed and almost spherical. They exhibited significant pH-sensitive properties and were fairly stable when diluted with a physiological solution. In conclusion, 2-ME-PSLs were successfully prepared and possessed a favorable pH sensitivity and good dissolution stability with a normal solution.

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Published
2019-12-04
How to Cite
He, S., Wang, B., Zhang, R., Zhou, H., & Yang, Q. (2019). Preparation and evaluation of 2-methoxyestradiol-loaded pH-sensitive liposomes. Brazilian Journal of Pharmaceutical Sciences, 55, e18204. https://doi.org/10.1590/s2175-97902019000118204
Section
Articles