Short-term high-fat diet affects macrophages inflammatory response, early signs of a long-term problem

  • Ed Wilson Santos University of Sao Paulo, School of Pharmaceutical Sciences https://orcid.org/0000-0002-7736-9645
  • Dalila Cunha Oliveira University of Sao Paulo, School of Pharmaceutical Sciences
  • Araceli Hastreiter University of Sao Paulo, School of Pharmaceutical Sciences
  • Graziela Batista Silva University of Sao Paulo, School of Pharmaceutical Sciences
  • Jackeline Soares de Oliveira Beltran University of Sao Paulo, School of Pharmaceutical Sciences
  • Marcelo Macedo Rogero University of Sao Paulo, School of Public Health
  • Ricardo Ambrósio Fock University of Sao Paulo, School of Pharmaceutical Sciences
  • Primavera Borelli University of Sao Paulo, School of Pharmaceutical Sciences
Keywords: High-fat diet/study, Mice, Macrophages, Metabolic syndrome, NF-kB

Abstract

Obesity is a chronic inflammatory disease that affects millions of people worldwide. Most studies observe the effects of a high-fat diet (HFD) in 10–12 weeks. This work investigated the effects induced by a HFD administered for 6 weeks on the nutritional status of mice and some aspects of the inflammatory response in mouse peritoneal macrophages. Male Swiss Webster mice, 2–3 months of age, were fed a control diet or HFD for 6 weeks. After this period, the mice were euthanized, and peritoneal macrophages were collected for immunoassays and assessment of biochemical parameters. A HFD was associated with increased cholesterol, insulin resistance, C-reactive protein (CRP), leptin, and serum resistin levels. Lipopolysaccharide (LPS)- stimulated adipocyte cultures of animals subjected to a HFD showed increased production of proinflammatory cytokines such as tumor necrosis factor alpha (TNF-α), interleukin-1 (IL-1), and interleukin-6 (IL-6). However, peritoneal macrophages of the HFD group showed no changes in the levels of these cytokines. LPS-stimulated peritoneal macrophages from HFD-treated animals showed a reduction in mRNA expression of TNF-α and IL-6, as well as a decrease in expression of the transcription factor nuclear factor-kappa B (NF-kB). In conclusion, HFD treatment for 6 weeks induces similar signs to metabolic syndrome and decreases the capacity of peritoneal macrophages to develop an appropriate inflammatory response to a bacterial component.

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Published
2019-12-04
How to Cite
Santos, E. W., Oliveira, D., Hastreiter, A., Silva, G., Beltran, J., Rogero, M., Fock, R., & Borelli, P. (2019). Short-term high-fat diet affects macrophages inflammatory response, early signs of a long-term problem. Brazilian Journal of Pharmaceutical Sciences, 55, e17561. https://doi.org/10.1590/s2175-97902019000117561
Section
Articles