Antileishmanial in vitro activity of essential oil from Myrciaria plinioides, a native species from Southern Brazil

  • Carla Kauffmann University of Vale do Taquari - Univates, Centro de Ciências Biológicas e da Saúde
  • Ana Caroline Giacomin University of Vale do Taquari - Univates, Centro de Ciências Biológicas e da Saúde
  • Kelen Arossi University of Vale do Taquari - Univates, Centro de Ciências Biológicas e da Saúde
  • Leandra Andressa Pacheco University of Vale do Taquari - Univates, Centro de Ciências Biológicas e da Saúde
  • Lucélia Hoehne University of Vale do Taquari - Univates, Centro de Ciências Exatas e Tecnológicas
  • Elisete Maria de Freitas University of Vale do Taquari - Univates, Centro de Ciências Biológicas e da Saúde
  • Gérzia Maria de Carvalho Machado Institute Oswaldo Cruz, Laboratory of Biochemistry of Trypanosomatid
  • Marilene Marcuzzo do Canto Cavalheiro Institute Oswaldo Cruz, Laboratory of Biochemistry of Trypanosomatid
  • Simone Cristina Baggio Gnoatto Federal University of Rio Grande do Sul, Faculty of Pharmacy
  • Eduardo Miranda Ethur University of Vale do Taquari - Univates, Centro de Ciências Exatas e Tecnológicas https://orcid.org/0000-0003-1731-3778
Keywords: Antileishmanial activity, Myrciaria plinioides, Myrtaceae, Leishmania amazonensis, Leishmania infantum

Abstract

In South American folk medicine members of the genus Myrciaria are used for the treatment of malaria, diarrhoea, asthma, inflammation and post-partum uterine cleansing. The aim of this work was to evaluate its antileishmanial properties (in vitro) of essential oil derived from leaves of Myrciaria plinioides D. Legrand, a plant species that is native in South of Brazil. The essential oil was obtained by hydro-distillation using fresh leaves of M. plinioides. The chemical composition of this essential oil (MPEO, M. plinioides essential oil) was determined by gas chromatography coupled to mass spectrometry (GC‑MS). MPEO was assayed in vitro for antileishmanial properties against promastigotes of Leishmania amazonensis and Leishmania infantum, and for cytotoxicity against murine peritoneal macrophages. The MPEO comprised 66 components and was rich in oxygenated sesquiterpenes (82.66%) containing spathulenol (21.12%) as its major constituent. The MPEO was effective against L. amazonensis with IC50 value of 14.16 ± 7.40 µg/mL, while against L. infantum the IC50 value was higher with 101.50 ± 5.78 µg/mL. The MPEO showed significant activity against L. amazonensis, and presented a selectivity index (SI) of 6.60. The results suggest that the essential oil from leaves of M. plinioides is a promising source for new antileishmanial agents against L. amazonensis.

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Published
2019-12-05
How to Cite
Kauffmann, C., Giacomin, A. C., Arossi, K., Pacheco, L., Hoehne, L., Freitas, E., Machado, G., Cavalheiro, M., Gnoatto, S., & Ethur, E. (2019). Antileishmanial in vitro activity of essential oil from Myrciaria plinioides, a native species from Southern Brazil. Brazilian Journal of Pharmaceutical Sciences, 55, e17584. https://doi.org/10.1590/s2175-97902019000217584
Section
Articles