Nanoemulsion containing caffeine for cellulite treatment: characterization and in vitro evaluation

  • Thamires Batello Freire University of São Paulo, Faculty of Pharmaceutical Sciences https://orcid.org/0000-0003-3655-3603
  • Michelli Ferrera Dario University of São Paulo, Faculty of Pharmaceutical Sciences
  • Otávio Gil Mendes University of São Paulo, Chemistry Institute
  • Andressa Costa de Oliveira University of São Paulo, Faculty of Pharmaceutical Sciences
  • Alberto Vetore Neto University of São Paulo, Faculty of Pharmaceutical Sciences
  • Dalva Lucia Araújo de Faria University of São Paulo, Chemistry Institute
  • Vânia Rodrigues Leite e Silva Federal University of São Paulo, Institute of Environmental Sciences
  • André Rolim Baby University of São Paulo, Faculty of Pharmaceutical Sciences
  • Maria Valéria Robles Velasco University of São Paulo, Faculty of Pharmaceutical Sciences
Keywords: Cellulite, Caffeine, Gynoid hydrolipodystrophy, Nanoemulsion, Permeation

Abstract

The Ginoide Hydrolipodystrophy (GHLD), commonly known as cellulite, occurs in 80-90% of the female population after the puberty period and comes from a metabolic modification in the cutaneous adipose tissue. Caffeine has been used in topical formulations due to its lipolytic action. We studied a nanoemulsion (F3) containing caffeine with two surfactants (oleth-3 and oleth-20) by emulsification method by phase inversion temperature inversion (PIT). The polydispersion indices (PDI) showed the reduced deviation of 0.1. The mean droplet size was ~ 40 nm. The evaluated constant of Ostwald, in the refrigerator condition was the most favorable during the stability test. In the In Raman spectroscopy assay, the caffeine bands found in F3 were compatible with those found in the caffeine solution (1337, 652.5 and 558.2 cm-1). There was no interaction of caffeine anhydrous with other ingredients in nanoemulsion. In the in vitro safety assay the result of 1.4 ranked the F3 as slightly irritating. In the natural membrane, cutaneous permeation test (human skin) permeate concentrations did not exceed the saturation concentration of the PBS buffer (48.96 μg/3 mL). The caffeine solution and F3 permeated statistically equal, but the nanoemulsion visually and sensorially improved the caffeine precipitation.

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Published
2019-12-06
How to Cite
Freire, T., Dario, M., Mendes, O., Oliveira, A., Vetore Neto, A., Faria, D., Silva, V., Baby, A., & Velasco, M. V. (2019). Nanoemulsion containing caffeine for cellulite treatment: characterization and in vitro evaluation. Brazilian Journal of Pharmaceutical Sciences, 55, e18236. https://doi.org/10.1590/s2175-97902019000218236
Section
Articles