Correlation between clinical signs of feline conjunctivitis and molecular detection of felid herpesvirus-1, feline calicivirus, chlamydophila felis and mycoplasma felis in cats from shelters in Rio de Janeiro

Authors

  • Natasha Baumworcel Universidade Federal Fluminense, Instituto Biomédico, Departamento de Microbiologia e Parasitologia http://orcid.org/0000-0002-7458-9474
  • Ana Maria Barros Soares Universidade Federal Fluminense, Faculdade de Veterinária
  • Sheila Bruna Silva Instituto Biomédico Universidade Federal Fluminense, Departamento de Microbiologia e Parasitologia
  • Núbia Karla Oliveira Almeida Universidade Federal Fluminense, Instituto de Matemática e Estatística, Departamento de Estatística
  • Tatiana Xavier de Castro Universidade Federal Fluminense, Instituto Biomédico, Departamento de Microbiologia e Parasitologia

DOI:

https://doi.org/10.11606/issn.1678-4456.bjvras.2017.104588

Keywords:

Ocular infection, Felis catus, Polymerase chain reaction, Upper respiratory tract disease, Eye

Abstract

Objectives: To perform molecular diagnosis of microbial agents (FHV-1, FCV, Mycoplasma felis, and Chlamydophila felis) in kittens with conjunctivitis and correlate the clinical signs with clinical severity. Material and Methods: A total of 108 conjunctival swab were collected from kittens without (G1; n = 40) and with (G2; n = 68) clinical signs of conjunctivitis. Animals from G2 group were scored from 1 (mild) to 4 (severe) according to the severity of conjunctivitis. All samples were submitted to PCR and RT-PCR. Results: FHV-1 was detected in 62/108 (57.4%) of samples, FCV in 40/108 (37.0%), M. felis in 11/108 (10.2%) and C. felis in 26/108 (24.1%). Mixed infections were detected in 39/108 (36.1%). In G1, 28/40 (70.0%) were positive for one or more agents, in G2, 58/68 (85.3%) were positive (P = 0.03). In 1, single infections by FHV-1were found in 21/40 (52.5%) samples, FCV in 2/40 (5.0%), C. felis in 1/40 (2.5%), and no pathogens were detected in 12/40 (30%) of samples, while mixed infections accounted for 29/40 (72.5%) of the cases. In G2, single FHV-1 infections were found in 31/68 (45.6%) samples, FCV in 10/68 (14.7 %), M. felis in 2/68 (3.0%) and C. felis also in 2/68 (3.0%), and no pathogens were detected in 10/68 (14.7%) samples, while mixed infections accounted for 36/68 (52.0%) of the cases. They were categorized as grade 1, 20/68 (29.4%), grade 2, 14/68 (20.6%), grade 3, 21/68 (30.9%) and grade 4, 13/68 (19.1%). The presence of FHV-1 and FCV is equally distributed among the four categories. More severe clinical signs, scores 3 and 4, are related to coinfections by C. felis and M. felis. Conclusions: FHV-1, FCV, C. felis and M. felis were identified in feline conjunctivitis. Co-infections are related to more severe cases of conjunctivitis. Molecular diagnosis is helpful to detect asymptomatic carriers and is a rapid and accurate method to determine the pathogen of feline conjunctivitis.

Downloads

Download data is not yet available.

Author Biography

Natasha Baumworcel, Universidade Federal Fluminense, Instituto Biomédico, Departamento de Microbiologia e Parasitologia

Departamento de Microbiologia e Parasitologia APlicada, Instituto Biomédico, Universidade Federal Fluminense

Downloads

Published

2017-05-19

How to Cite

Baumworcel, N., Soares, A. M. B., Silva, S. B., Almeida, N. K. O., & Castro, T. X. de. (2017). Correlation between clinical signs of feline conjunctivitis and molecular detection of felid herpesvirus-1, feline calicivirus, chlamydophila felis and mycoplasma felis in cats from shelters in Rio de Janeiro. Brazilian Journal of Veterinary Research and Animal Science, 54(1), 18-26. https://doi.org/10.11606/issn.1678-4456.bjvras.2017.104588

Issue

Section

FULL ARTICLE

Most read articles by the same author(s)