In vitro embryo development from bovine oocytes maintained in follicular fluid or TCM-hepes

  • Denis Faustino Alves Universidade Federal de Santa Maria, Centro de Ciências Rurais, Departamento de Clínica de Grandes Animais, Laboratório de Embriologia Animal, Santa Maria, RS
  • Lucio Pereira Rauber Universidade Federal de Santa Maria, Centro de Ciências Rurais, Departamento de Clínica de Grandes Animais, Laboratório de Embriologia Animal, Santa Maria, RS
  • Fernanda Bastos Rubin Universidade Federal de Santa Maria, Centro de Ciências Rurais, Departamento de Clínica de Grandes Animais, Laboratório de Embriologia Animal, Santa Maria, RS
  • Mari Lourdes Bernardi Universidade Federal do Rio Grande do Sul, Departamento de Zootecnia, Porto Alegre, RS
  • Diogenes Dezen Universidade Federal de Santa Maria, Centro de Ciências Rurais, Departamento de Clínica de Grandes Animais, Laboratório de Embriologia Animal, Santa Maria, RS
  • Carlos Antônio Mondino Silva Universidade Federal de Santa Maria, Centro de Ciências Rurais, Departamento de Clínica de Grandes Animais, Laboratório de Embriologia Animal, Santa Maria, RS
  • Mara Iolanda Batistella Rubin Universidade Federal de Santa Maria, Centro de Ciências Rurais, Departamento de Clínica de Grandes Animais, Laboratório de Embriologia Animal, Santa Maria, RS
Keywords: Bovine, Oocytes, Embryo, Maturation

Abstract

In order to evaluate the effect of a transport medium on the rate of in vitro embryonic development, 1381 Cumulus-oocyte Complexes (COC) were obtained by aspiration of 2-8mm diameter follicles witch were randomly divided in 4 treatment groups. The Control group was formed by oocytes matured in modified TCM-199 for 24h, incubated at 39°C and 5,00% CO2 with saturated humidity. The group 1 (WB24h), included oocytes matured in 1.0mL tubes containing TCM-HEPES (5.95mg/mL), in water bath (WB) at 39°C for 24h. The group 2 (FFb6C18h), included oocytes kept in bovine follicular fluid (FFb) for 6h at 30°C followed by a period of 18h maturation under the same conditions as the Control group and with the oocytes maintained in FFb followed by 18h IVM under the same conditions as the group 1, group 3 (FFb6WB18h). Fertilization was performed in FERT-TALP for 18h. Zygotes were cultured in SOFaaci under mineral oil within gasified bags. The cleavage rate differed (P<0.05) between the Control and FFb6BM18h groups. However, there was no difference on the D7 and D9 blastocyst rates and on the percentage of blastocyst ecloded. It was concluded that it is possible to maintain the oocytes in FFb for 6h at 30°C before 18h IVM, or to proote the transport and maturation of the oocytes for 24h, in TCM-HEPES and water-bath at 39°C, without compromising embryonic development. The simplification of MIV showed in this experiment through of tubes (1.0mL) replete with TCM-HEPES and holding in water bath at 39°C, could be a viable and practice for the bovine programs of OPU/PIV.

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Published
2003-01-01
How to Cite
Alves, D., Rauber, L., Rubin, F., Bernardi, M., Dezen, D., Silva, C., & Rubin, M. (2003). In vitro embryo development from bovine oocytes maintained in follicular fluid or TCM-hepes. Brazilian Journal of Veterinary Research and Animal Science, 40(4), 279-286. https://doi.org/10.1590/S1413-95962003000400007
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