Embryo development of bovine oocytes held in follicular fluid from bovine follicles of different diameters

  • Lucio Pereira Rauber Universidade Federal de Santa Maria, Centro de Ciências Rurais, Departamento de Clínica de Grandes Animais, Laboratório de Embriologia Animal, Santa Maria, RS
  • Denis Faustino Alves Universidade Federal de Santa Maria, Centro de Ciências Rurais, Departamento de Clínica de Grandes Animais, Laboratório de Embriologia Animal, Santa Maria, RS
  • Giuliano Moraes Figueiró Universidade Federal de Santa Maria, Centro de Ciências Rurais, Departamento de Clínica de Grandes Animais, Laboratório de Embriologia Animal, Santa Maria, RS
  • Daniela dos Santos Brum Universidade Federal de Santa Maria, Centro de Ciências Rurais, Departamento de Clínica de Grandes Animais, Laboratório de Embriologia Animal, Santa Maria, RS
  • Tiago Fernando Hilgert Universidade Federal de Santa Maria, Centro de Ciências Rurais, Departamento de Clínica de Grandes Animais, Laboratório de Embriologia Animal, Santa Maria, RS
  • Mari Lourdes Bernardi Universidade Federal do Rio Grande do Sul, Departamento de Zootecnia, Porto Alegre, RS
  • Carlos Antônio Mondino Silva Universidade Federal de Santa Maria, Centro de Ciências Rurais, Departamento de Clínica de Grandes Animais, Laboratório de Embriologia Animal, Santa Maria, RS
  • Mara Iolanda Batistella Rubin Universidade Federal de Santa Maria, Centro de Ciências Rurais, Departamento de Clínica de Grandes Animais, Laboratório de Embriologia Animal, Santa Maria, RS
Keywords: Transport, Follicular diameter, Maturation, Meiosis

Abstract

Bovine oocytes have been maintained in the follicular fluid to be transported and to increase their competence, before maturation. Eight hundred eighty-one (881) oocytes, aspirated from bovine slaughterhouse ovaries, were used to evaluate the effect of holding bovine oocytes in follicular fluid (FF) of bovine follicles of different diameters on the rate of embryo development. The oocytes were randomly distributed in four treatments with seven replicates each: The control group (n=217) was constituted by oocytes matured for 24h in modified TCM-199 with Estrus Mare Serum (EMS), pyruvate and rFSH-h in incubator with 5,00% CO2, 39°C and saturated humidity. In the FFsmall group (3 to 5mm follicles; n=216), the oocytes were held for 6h in follicular fluid at 30°C and matured for 18h in the same conditions of the Control-group. The oocytes of the FFmedium group (5,1-8mm follicles; n=226) and of the FFlarge group (>;8,1mm follicles; n=222) were held in follicular fluid and matured like FFsmall. Fertilization was accomplished during 18h and, after this, the zygotes were cultured for 8 days in SOFaaci medium + 5,00% EMS in incubator at 39°C using plastic bags gasified with 5,00%CO2, 5,00%O2 and 90,00%N2. FFsmall oocytes produced a lower (P;0,05) between the groups. Follicular fluid of medium and large follicles could be used to hold for 6h at 30°C bovine oocytes before their maturation for 18h.

Downloads

Download data is not yet available.
Published
2003-01-01
How to Cite
Rauber, L., Alves, D., Figueiró, G., Brum, D., Hilgert, T., Bernardi, M., Silva, C., & Rubin, M. (2003). Embryo development of bovine oocytes held in follicular fluid from bovine follicles of different diameters. Brazilian Journal of Veterinary Research and Animal Science, 40(3), 169-177. https://doi.org/10.1590/S1413-95962003000300002
Section
UNDEFINIED