Decontamination of genetically modified mice strains by embryo transfer for obtaining SPF colonies in a Brazilian animal facility

  • Ana Tada Fonseca Brasil Antiorio Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Patologia https://orcid.org/0000-0002-8186-3013
  • Sílvia Maria Gomes Massironi Universidade de São Paulo, Instituto de Ciências Biomédicas, Biotério de Camundongos Isogênicos, Departamento de Imunologia
  • Rosália Regina de Luca Universidade de São Paulo, Instituto de Ciências Biomédicas, Biotério de Camundongos Isogênicos, Departamento de Imunologia
  • Márcio Augusto Caldas Rocha Carvalho Universidade de São Paulo, Instituto de Ciências Biomédicas, Biotério de Camundongos Isogênicos, Departamento de Imunologia
  • Vanessa Yamamoto Tambellini Universidade de São Paulo, Instituto de Ciências Biomédicas, Biotério de Camundongos Isogênicos, Departamento de Imunologia
  • Danilo Wadt Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Patologia
  • Juliana Bortolatto Universidade de São Paulo, Faculdade de Medicina, Laboratório de Controle de Qualidade Genética e Sanitária do Centro de Bioterismo
  • Joana Bom Instituto Gulbenkian de Ciência
  • Claudia Madalena Cabrera Mori Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Patologia
Keywords: Strains rederivation, Animal reception, Cleaning colonies, Assisted reproduction, Mouse embryos

Abstract

The introduction of new strains of mice in specific pathogen-free (SPF) animal facilities should be performed carefully to avoid breaking sanitary barriers. To meet this need, animals should be rederived to reduce infection risk and thus avoid research interference caused by loss of animal health status and welfare. The objective of this study was to implement mice embryo transfer in the laboratory mouse facility of the Department of Immunology at the Institute of Biomedical Sciences/University of São Paulo, Brazil. Embryo transfers were performed to rederive genetically modified mouse strains with undefined sanitary status, received from different research and educational institutions. Fertilized eggs at two-cell stage were obtained by natural means and transferred into the oviducts of SPF pseudo-pregnant female mice. All surgical procedures were performed under aseptic conditions. A total of 625 embryos were transferred into the
recipients. 148 pups were born, of which 140 were reared. Viruses, bacteria and intestinal protozoa were eliminated using this technique. The improvement in the microbiological status of mice allowed their expansion in our SPF facility. With these results, we can stimulate the use of embryo transfer technique between rodent facilities in Brazil and thus encourage the distribution of better models to our scientific community.

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Published
2019-06-26
How to Cite
Antiorio, A., Massironi, S., Luca, R., Carvalho, M., Tambellini, V., Wadt, D., Bortolatto, J., Bom, J., & Mori, C. (2019). Decontamination of genetically modified mice strains by embryo transfer for obtaining SPF colonies in a Brazilian animal facility. Brazilian Journal of Veterinary Research and Animal Science, 56(1), e143588. https://doi.org/10.11606/issn.1678-4456.bjvras.2019.143588
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