Seasonal variation of propolis from southern Brazil:

phytochemical screening, antimicrobial activity, and effects on bovine mammary epithelial cells

  • Samira de Aquino Leite Fiordalisi Universidade Federal de Santa Catarina, Centro de Ciências Agrárias, Programa de Pós-Graduação em Agroecossistemas
  • Luciana Aparecida Honorato Universidade Federal de Santa Catarina, Centro de Ciências Biológicas
  • Shirley Kuhnen Universidade Federal de Santa Catarina, Centro de Ciências Agrárias, Departamento de Zootecnia e Desenvolvimento Rural, Laboratório de Morfofisiologia e Bioquímica Animal https://orcid.org/0000-0002-1882-5241
Keywords: Bovine mastitis, Brazilian propolis, MAC-T cells, Staphylococcus aureus

Abstract

Previous studies have reported on the phytotherapeutic potential of propolis from southern Brazil (Urupema, Santa Catarina State), in particular, its efficacy in the treatment of bovine mastitis. The present study evaluates the effect of seasonal variation on the chemical composition of propolis from southern Brazil and its resultant antimicrobial and cytotoxic activities in the context of treating bovine mastitis. Antimicrobial activity against Staphylococcus aureus was assessed, along with the cytotoxicity and induction of apoptosis in MAC-T bovine mammary epithelial cells. With the exception of spring propolis, the flavonoid quercetin was the main compound present in all samples. The minimum inhibitory concentration (MIC) of propolis against S. aureus from mastitic milk was 140 μg/mL for samples collected during the spring, autumn, and winter seasons, but 280 μg/mL for samples collected in summer. For MAC-T cells, the spring propolis extract was more toxic with an IC50 of 120 μg/mL. However, for 120 μg/mL of spring propolis extract, only 0.77% of necrotic and 37% of apoptotic MAC-T cells were found, respectively. Nonetheless, the induction of cell death
through apoptosis by propolis extract suggests less severe damage to bovine mammary glands. Moreover, only negligible seasonal variation was found in the chemical composition of propolis from southern Brazil, and no biological activities were determined to be harmful. Therefore, this propolis shows promise as an alternative to commercial antimicrobials in the control of bovine mastitis, offering support for organic milk production.

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Published
2019-07-05
How to Cite
Fiordalisi, S., Honorato, L., & Kuhnen, S. (2019). Seasonal variation of propolis from southern Brazil:. Brazilian Journal of Veterinary Research and Animal Science, 56(1), e149146. https://doi.org/10.11606/issn.1678-4456.bjvras.2019.149146
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