Evidence of in utero infection by Mycobacterium avium subsp. paratuberculosis using Multiple-Locus Variable-number tandem-repeat Analysis:

first report in Argentina

  • Brenda Vasini Rosell Conselho Nacional de Pesquisa Científica e Tecnológica; Estação Experimental Agrícola INTA, Laboratório de Bacteriologia
  • Bernardo Lagleyze Autônomo
  • Claudia Morsella Estação Experimental Agrícola INTA, Laboratório de Bacteriologia
  • Laura Mendez Estação Experimental Agrícola INTA, Laboratório de Bacteriologia
  • Florencia Bresky Estação Experimental Agrícola INTA, Laboratório de Bacteriologia; Universidade Nacional de Mar del Plata, Faculdade de Ciências Agrícolas
  • Andrea Gioffré Conselho Nacional de Pesquisa Científica e Tecnológica; Instituto de Agrobiotecnologia e Biologia Molecular
  • Fernando Paolicchi Estação Experimental Agrícola INTA, Laboratório de Bacteriologia; Universidade Nacional de Mar del Plata, Faculdade de Ciências Agrícolas https://orcid.org/0000-0002-7481-2285
Keywords: Paratuberculosis, In utero infection, Molecular diagnosis, Bovine fetus

Abstract

A pregnant heifer with an advanced clinical stage of paratuberculosis was reported in a herd in Argentina. Thus, the animal was euthanized and samples of organs of the cow and its fetus was taken and cultured for bacteriology in specific medium. Tissues were analyzed by histopathology (hematoxylin-eosin and Ziehl-Neelsen staining). Histopathological analysis of the cow’s samples revealed the presence of lesions consistent with paratuberculosis, and Ziehl-Neelsen staining revealed the presence of acid-fast bacilli, whereas the fetal tissues showed absence of lesions but the presence of acid-fast bacilli by Ziehl-Neelsen staining. After growing in specific medium, colonies in tissues from both cow and fetus were positive for IS900-PCR, confirming the presence of Mycobacterium avium subsp. paratuberculosis (MAP). Finally, the isolates were typed by Multiple-Locus Variable-number tandem-repeat Analysis (MLVA), which confirmed
the epidemiological link between them. This study is the first in Argentina to report the detection of MAP that shares an identical MLVA type in a pregnant cow and its fetus. The results of this study are consistent with previous reports and highlight the intra-uterine transmission of MAP as an important source of infection within herds.

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Author Biographies

Brenda Vasini Rosell, Conselho Nacional de Pesquisa Científica e Tecnológica; Estação Experimental Agrícola INTA, Laboratório de Bacteriologia

Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), Buenos Aires, Argentina.

 Laboratorio de Bacteriología, Estación Experimental Agropecuaria INTA, Balcarce, Argentina. 

Claudia Morsella, Estação Experimental Agrícola INTA, Laboratório de Bacteriologia

 Laboratorio de Bacteriología, Estación Experimental Agropecuaria INTA, Balcarce, Argentina. 

Laura Mendez, Estação Experimental Agrícola INTA, Laboratório de Bacteriologia

 Laboratorio de Bacteriología, Estación Experimental Agropecuaria INTA, Balcarce, Argentina. 

Florencia Bresky, Estação Experimental Agrícola INTA, Laboratório de Bacteriologia; Universidade Nacional de Mar del Plata, Faculdade de Ciências Agrícolas

Facultad de Ciencias Agrarias, Universidad Nacional de Mar del Plata

Comisión de Investigaciones Científicas (CIC), Argentina

Laboratorio de Bacteriología, Estación Experimental Agropecuaria INTA, Balcarce, Argentina. 

Andrea Gioffré, Conselho Nacional de Pesquisa Científica e Tecnológica; Instituto de Agrobiotecnologia e Biologia Molecular

 Instituto de Agrobiotecnología y Biología Molecular (IABIMO), Instituto Nacional de Tecnología Agropecuaria (INTA).

Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), Buenos Aires, Argentina. 

Fernando Paolicchi, Estação Experimental Agrícola INTA, Laboratório de Bacteriologia; Universidade Nacional de Mar del Plata, Faculdade de Ciências Agrícolas

Laboratorio de Bacteriología, Estación Experimental Agropecuaria INTA, Balcarce, Argentina.

Facultad de Ciencias Agrarias, Universidad Nacional de Mar del Plata 

Published
2020-04-27
How to Cite
Vasini Rosell, B., Lagleyze, B., Morsella, C., Mendez, L., Bresky, F., Gioffré, A., & Paolicchi, F. (2020). Evidence of in utero infection by Mycobacterium avium subsp. paratuberculosis using Multiple-Locus Variable-number tandem-repeat Analysis:. Brazilian Journal of Veterinary Research and Animal Science, 57(1), e161653. https://doi.org/10.11606/issn.1678-4456.bjvras.2020.161653
Section
CASE REPORT

Funding data