Experimental skin wound treatment with Copaifera langsdorffii Desf Kuntze (Leguminosae) extract and oil-resin in horses

  • Débora Perrone Kauer Universidade Estadual Paulista, Faculdade de Medicina Veterinária e Zootecnia
  • Juliana de Moura Alonso Universidade Estadual Paulista, Faculdade de Medicina Veterinária e Zootecnia
  • Lucas Fernando Sergio Gushiken Universidade Estadual Paulista, Instituto de Biociências
  • Marivane Lemos Universidade de São Paulo, Faculdade de Ciências Farmacêuticas de Ribeirão Preto
  • Carlos Roberto Padovani Universidade Estadual Paulista, Instituto de Biociências
  • Celso Antonio Rodrigues Universidade Estadual Paulista, Faculdade de Medicina Veterinária e Zootecnia
  • Ana Liz Garcia Alves Universidade Estadual Paulista, Faculdade de Medicina Veterinária e Zootecnia
  • Marcos Jun Watanabe Universidade Estadual Paulista, Faculdade de Medicina Veterinária e Zootecnia
  • Jairo Kenupp Bastos Universidade de São Paulo, Faculdade de Ciências Farmacêuticas de Ribeirão Preto
  • Cláudia Helena Pellizzon Universidade Estadual Paulista, Instituto de Biociências
  • Carlos Alberto Hussni Universidade Estadual Paulista, Faculdade de Medicina Veterinária e Zootecnia https://orcid.org/0000-0001-5421-2904
Keywords: Copaiba, Healing, Phytotherapy

Abstract

Copaifera langsdorffii is a Brazilian native plant that is used for wound healing or as an anti-inflammatory agent. Non-healing wounds are an important health problem, particularly in horses, because they can cause the animal invalidity or even lead to death. In addition, horses respond to skin wounds with chronic inflammatory response and intense wound granulation, thereby delaying the healing process. By this way, our aim was to evaluate the healing potential of Copaifera langsdorffii hydroalcoholic extract (HE) and oil-resin creams (OR) in horse skin wounds. Four wounds were performed bilaterally in the lumbar region of six horses with a 2 cm punch and treated daily with the respective treatments: saline solution, vehicle, 10% HE creams or 10% OR cream. Daily planimetry analyses were performed to measure the wound area and clinical parameters. In four different experimental periods (3, 7, 14 and 21 d), wound biopsies were removed and used for microscopic analyses. SS wounds presented a significant small area at day 3 and 7, OR wounds presented significant small area in comparison with HE at 14 d, and no significant difference was observed between treatments at 21 d. A better microscopic and clinical healing activity of HE and OR was identified in comparison with the controls. The OR group showed better healing quality, specifically after 7 d of treatment. Therefore, Copaifera langsdorffii formulations demonstrated their wound healing potential in horse skin lesions, exhibiting an improvement of the macro- and microscopic parameters.

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References

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Published
2020-09-15
How to Cite
Kauer, D., Alonso, J., Gushiken, L. F. S., Lemos, M., Padovani, C. R., Rodrigues, C. A., Alves, A. L., Watanabe, M., Bastos, J., Pellizzon, C. H., & Hussni, C. A. (2020). Experimental skin wound treatment with Copaifera langsdorffii Desf Kuntze (Leguminosae) extract and oil-resin in horses. Brazilian Journal of Veterinary Research and Animal Science, 57(3), e166095. https://doi.org/10.11606/issn.1678-4456.bjvras.2020.166095
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