Frequency of leptospirosis in horses in Manaus and metropolitan region in Amazonas State, Brazil

Authors

  • Isadora Karolina Freitas de Sousa Instituto Federal de Educação, Ciência e Tecnologia do Amazonas
  • Rebeca Larissa Castro Silva Instituto Federal de Educação, Ciência e Tecnologia do Amazonas
  • Rejane dos Santos Sousa Universidade Federal do Sul e Sudeste do Pará, Instituto de Estudos do Trópico Úmido
  • Claudia Elisa Martins Vieira Universidade Federal do Amazonas, Programa de Pós-graduação em Ciência Animal
  • Sérgio de Melo Universidade Federal do Oeste do Pará, Programa de Pós-graduação em Sociedade, Natureza e Desenvolvimento
  • Geórgea Portella Quevedo Universidade Federal de Santa Maria, Laboratório de Leptospirose
  • Ana Eucares Von Laer Universidade Federal de Santa Maria, Laboratório de Leptospirose
  • Luciane Teresinha Lovato Universidade Federal de Santa Maria, Laboratório de Leptospirose
  • Alexandre Alberto Tonin Instituto Federal de Educação, Ciência e Tecnologia do Amazonas

DOI:

https://doi.org/10.11606/issn.1678-4456.bjvras.2020.172607

Keywords:

Amazonas, Metropolitan region, Epidemiology, Equine, Leptospira

Abstract

The occurrence of leptospirosis is significantly higher in the tropical climate than in temperate regions, especially in periods with high rainfall levels. In horses, leptospirosis can range from subclinical to asymptomatic but maintains an important role in the transmission of leptospirosis by eliminating the agent in the environment. Regarding horses, the Amazonas herd increased by 30% between 2004 and 2013. Thus, the study aimed to determine the serological prevalence of leptospirosis in horses in Manaus and metropolitan regions, in the State of Amazonas, Brazil as well as to assess the main serogroups involved in the infections of these animals. For this purpose, from August 2018 to July 2019, serum from 198 horses was evaluated through the microscopic agglutination test with a panel of 10 serogroups. As a result, 92 horses (44.46%) were found to be positive for one or more Leptospira serovars, with the highest prevalence of serogroups Icterohaemorrhagiae and Pyrogenes. Therefore, with the increase in the equine herd in the region and, consequently, greater interaction between breeders and animals, the identification of reagents to at least one serovar of Leptospira spp. signals the possible existence of reservoirs of pathogenic strains for other animals and man.

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References

Aguiar DM, Cavalcante GT, Marvulo MFV, Silva JCR, Pinter A, Vasconcellos SA, Morais ZM, Labruna MB, Camargo LMA, Gennari SM. Fatores de risco associados à ocorrência de anticorpos anti-Leptospira spp. em cães do município de Monte Negro, Rondônia, Amazônia Ocidental Brasileira. Arq Bras Med Vet Zootec. 2007;59(1):70. http://doi.org/10.1590/S0102-09352007000100012.

Alves JRA, Oliveira KDS, Costa DF, Fernandes LG, Higino SSS, Alves CJ, Santos CSAB, Azevedo SS. Caracterização epidemiológica da leptospirose em equinos do estado de Pernambuco, nordeste do Brasil. Arq Inst Biol. 2016;83:1-5. http://doi.org/10.1590/1808-1657001032014.

Brasil. Ministério da Agricultura, Pecuária e Abastecimento. Revisão do estudo do complexo do agronegócio do cavalo. Brasília: MAPA; 2016. 54 p.

Cole JR Jr, Sulzer CR, Pursell AR. Improved microtechnique for the leptospiral microscopic agglutination test. Appl Microbiol. 1973;25(6):976-80. http://doi.org/10.1128/AEM.25.6.976-980.1973. PMid:4736794.

Donahue M. Equine leptospirosis. Equine Dis Quarterl. 1995;3(4):4-5.

Faria MT, Calderwood MS, Athanazio DA, McBride AJA, Hartskeerl RA, Pereira MM, Ko AI, Reis MG. Carriage of Leptospira interrogans among domestic rats from an urban setting highly endemic for leptospirosis in Brazil. Acta Trop. 2008;108(1):1-5. http://doi.org/10.1016/j.actatropica.2008.07.005. PMid:18721789.

Farias DK. Aspectos soroepidemiológicos e laboratoriais da leptospirose em equinos [tese]. Lages: Programa de Pós-graduação em Ciência Animal, Universidade do Estado de Santa Catarina; 2019.

Favero ACM, Pinheiro SR, Vasconcellos AS, Morais ZM, Ferreira F, Ferreira Neto JS. Sorovaresde leptospiras predominantes em exames sorológicos de bubalinos, ovinos, caprinos, eqüinos, suínos e cães de diversos estados brasileiros. Cienc Rur. 2002;32:613-619. http://doi.org/10.1590/S0103-84782002000400011.

Galton MM, Sulzer CR, Santa Rosa CA, Fields MJ. Application of a microtechnique to the agglutination test for leptospiral antibodies. Appl Microbiol. 1965;13(1):81-5. http://doi.org/10.1128/AEM.13.1.81-85.1965. PMid:14264852.

Hamond C, Martins G, Lawson-Ferreira R, Medeiros MA, Lilenbaum W. The role of horses in the transmission of leptospirosis in an urban tropical area. Epidemiol Infect. 2013;141(1):33-5. http://doi.org/10.1017/S0950268812000416. PMid:22417781.

Hashimoto VY, Gonçalves DD, Silva FG, Oliveira RC, Alves LA, Reichmann P, Muller EE, Freitas JC. Occurence of antibodies against Leptospira spp. in horses of the urban area of Londrina, Paraná. Brasil. Rev Inst Med Trop São Paulo. 2007;49(5):327-30. http://doi.org/10.1590/S0036-46652007000500010. PMid:18026641.

Hong CB, Donahue JM, Giles JRRC Jr, Petrites-Murphy MB, Poonacha KB, Roberts AW, Smith BJ, Tramontin RR, Tuttle PA, Swerczek TW. Equine abortion and stillbirth in central Kenutucky during 1988 and 1989 following seasons. J Vet Diagn Invest. 1993;5(4):560-6. http://doi.org/10.1177/104063879300500410. PMid:8286455.

Hunter P, Herr S. Leptospirosis. In: Cortez JAW, Thomson GR, Tustin RC, editors. Infectious diseases of Livestock. Oxford: Oxford University; 1994. p. 997-1008.

Jesus MS, Silva LA, Lima KMS, Fernandes OCC. Cases distribution of leptospirosis in City of Manaus, State of Amazonas, Brazil, 2000-2010. Rev Soc Bras Med Trop. 2012;45(6):713-6. http://doi.org/10.1590/S0037-86822012000600011. PMid:23295874.

Langoni H, Kuribara IY, Correa APFL, Ullmann LS, Sánchez GP, Lucheis SB. Anti-leptospirosis agglutinins in Brazilian capybaras (Hydrochoerus hydrochaeris). J Venom Anim Toxins Incl Trop Dis. 2016;22(1):4. http://doi.org/10.1186/s40409-016-0059-6. PMid:26819575.

Langoni H, Silva AV, Pezerico SB, Lima VY. Anti-Leptospire agglutinins in equine sera, from São Paulo, Goiás and Mato Grosso do Sul, Brazil, 1996-2001. J Venom Anim Toxins. 2004;10(3):207-18. http://doi.org/10.1590/S1678-91992004000300003.

Levett PN. Leptospirosis. Clin Microbiol Rev. 2001;14(2):296-326. http://doi.org/10.1128/CMR.14.2.296-326.2001. PMid:11292640.

Lilenbaum W. Leptospirosis on animal reproduction: IV. Serological findings in mares from six farms in Rio de Janeiro, Brazil (1993-1996). Braz J Vet Res Anim Sci. 1998;35(2):61-3. http://doi.org/10.1590/S1413-95961998000200002.

Magalhães DF, Silva JA, Moreira EC, Wilke VML, Haddad JPA, Meneses JNC. Prevalência de aglutininas anti-Leptospira interrogans em cães de Belo Horizonte, Minas Gerais, 2001 a 2002. Arq Bras Med Vet Zootec. 2006;58(2):167-74. http://doi.org/10.1590/S0102-09352006000200004.

Megid M, Ribeiro MG, Paes AC. Doenças infeciosas em animais de produção e de companhia. Rio de Janeiro: Roca; 2016.

Miot HA. Tamanho da amostra em estudos clínicos e experimentais. J Vasc Bras. 2011;10(4):275-8. http://doi.org/10.1590/S1677-54492011000400001.

Monte LG, Jorge S, Xavier MA, Leal FMA, Amaral MG, Seixas FK, Dellagostin AO, Hartleben CP. Molecular characterization of virulent Leptospira interrogans serogroup Icterohaemorrhagiae isolated from Cavia aperea. Acta Trop. 2013;126(2):164-6. http://doi.org/10.1016/j.actatropica.2013.02.009. PMid:23435256.

Morais CCG, Kuroda RBS, Pinho APVB, Ywasaki F, Meneses AMC, Martins AV, Amaral JM Jr. Pesquisa de anticorpos para sorovares de Leptospira interrogans patogênicas em equídeos criados na ilha de Algodoal, Estado do Pará. Rev Cienc Agrária. 2010;53(2):188-94. http://doi.org/10.4322/rca.2011.027.

Pinheiro HH, Silveira W, Oliveira VC. Pesquisas de aglutininas anti- leptospiras em soros eqüinos abatidos no frigorífico Xavante – Araguari, MG. Hora Vet. 1985;5(27):42-4.

Pinna M, Martins G, Freire I, Lilenbaum W. Seropositivity to Leptospira interrogans serovar Bratislava associated to reproductive problems without significant biochemical or hematological alterations in horses. Cienc Rural. 2010;40(10):2214-7. http://doi.org/10.1590/S0103-84782010005000178.

Pires JAS No, Hesse F, Oliveira MAM. Leptospirose eqüina: aspectos clínicos, tratamento, prevenção e levantamento sorológico. Vet Foco. 2005;2(2):165.

Querino AMV, Delbem ÁCB, Oliveira RC, Silva FG, Müller EE, Freire RL, Freitas JC. Fatores de risco associados à leptospirose em cães do município de Londrina-PR. Semina. 2003;24(1):27-34. http://doi.org/10.5433/1679-0359.2003v24n1p27.

Radostits OM, Gay CC, Hinchcliff KW, Constable PD. Veterinary medicine. 10th ed. London: Elsevier Saunders; 2007. 2156 p.

Ribeiro TMP. Soroepidemiologia da infecção por Leptospira spp. em equinos do município de Rorainópolis, estado de Roraima, Brasil [dissertação]. Goiânia: Escola de Veterinária e Zootecnia, Universidade Federal de Goiás; 2015.

Santa Rosa CA, Sulzer CR, Yanaguita RM, Silva AS. Leptospirosis in wildlife in Brazil: Isolation of serovars Canicola, Pyrogenes and Grippotyphosa. Int J Zoonoses. 1980;7:40-3.

Schalm OW. Clinical significance of plasma protein concentration. J Am Vet Med Assoc. 1970;157(11):1672-3. PMid:5530370.

Silva LA, Lima KMS, Fernandes OCC, Balassiano IT, Avelar KE, Jesus MS. Seroprevalence of and risk factors for leptospirosis in the City of Manaus, State of Amazonas, Brazil. Rev Soc Bras Med Trop. 2016;49(5):628-31. http://doi.org/10.1590/0037-8682-0115-2016. PMid:27812660.

Timoney JF, Kalimuthusamy N, Velineni S, Donahue JM, Artiushin SC, Fettinger M. A unique genotype of Leptospira interrogans sorovar Pomona type Kennewicki is associated with equine abortion. Vet Microbiol. 2011;150(3-4):349-53. http://doi.org/10.1016/j.vetmic.2011.02.049. PMid:21450416.

Tonin AA, Silva ASD, Azevedo MID, França RT, Paim FC, Schaefer PC, Martins JLR, Badke MRT, Lopes STDA. Hematologic and biochemical alterations in Wistar rats experimentally infected by Leptospira interrogans. Comp Clin Pathol. 2012;21(5):833-8. http://doi.org/10.1007/s00580-011-1186-7.

World Health Organization – WHO. Leptospirosis [Internet]. Geneva: WHO; 2012 [cited 2020 July 20]. Available from: http://www.wpro.who.int/mediacentre/factsheets/fs_13082012_leptospirosis/en/

Yan W, Faisal SM, Divers T, McDonough SP, Akey B, Chang YF. Experimental Leptospira interrogans sorovar Kennewicki infection of horses. J Vet Intern Med. 2010;24(4):912-7. http://doi.org/10.1111/j.1939-1676.2010.00507.x. PMid:20649749.

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Published

2020-12-01

How to Cite

Sousa, I. K. F. de, Silva, R. L. C., Sousa, R. dos S., Vieira, C. E. M., Melo, S. de, Quevedo, G. P., Von Laer, A. E., Lovato, L. T., & Tonin, A. A. (2020). Frequency of leptospirosis in horses in Manaus and metropolitan region in Amazonas State, Brazil. Brazilian Journal of Veterinary Research and Animal Science, 57(4), e172607. https://doi.org/10.11606/issn.1678-4456.bjvras.2020.172607

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