Biological and immunological studies of five Brazilian rabies virus isolates

  • Fernanda Bernardi Agência Paulista de Tecnologia dos Agronegócios, Instituto Biológico, Laboratório de Anatomia Patológica, São Paulo, SP
  • Antonio Albério de Barros Gomes Universidade Federal de Campina Grande, Departamento de Medicina Veterinária, Campina Grande, PB
  • Fumio Honmai Ito Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Medicina Veterinária Preventiva e Saúde Animal, São Paulo, SP
  • Leonardo José Richtzenhain Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Medicina Veterinária Preventiva e Saúde Animal, São Paulo, SP
  • Rodrigo Martins Soares Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Medicina Veterinária Preventiva e Saúde Animal, São Paulo, SP
  • Marcos Bryan Heinemann Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Medicina Veterinária Preventiva e Saúde Animal, São Paulo, SP
  • Adriana Cortez Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Medicina Veterinária Preventiva e Saúde Animal, São Paulo, SP
  • Sidney Mioshy Sakamoto Escola Superior de Agricultura de Mossoró, Mossoró, RN
  • Paulo César Maiorka Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Patologia, São Paulo, SP
Keywords: Rabies virus, Biological and immunological studies, Brazilian strains

Abstract

Aspects of biological behavior of Brazilian rabies virus isolated from canine, bovine, equine, vampire and insectivorous bats were studied in mice. The oral infection occurred in mice fed with infected brain of insectivours bat (8.82%), canine (8.57%) and equine (3.03%). The mean period of incubation to all the isolates was 6 days after mice intracerebral inoculation, however, symptoms were variable, since hyperexcitability (canine sample), progressive paralysis of lower limbs and prolonged clinical course until death (equine sample), and mice without clinical signs before death (insectivorous bat). By immunohistochemistry IFN was detected in brains of mice inoculated with bovine and insectivorous bat samples, TNF and iNOS were detected in brains of those inoculated with insectivorous bat, bovine and canine samples, and positive GFAP astrocytes were found in all five samples. Two commercial inactivated rabies vaccines, one imported (vaccine 1) and another manufactured in Brazil (vaccine 2) were compared to evaluate their efficacy to protect against experimental rabies infection in mice, through the NIH and the CDC potency tests, using these street isolates as challenge virus. There was no statistical significant difference between the efficaccy of both vaccines, when comparing the same potency test and challenge virus strain suggesting no need to produce specific vaccines with street isolates.

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Published
2005-08-01
How to Cite
Bernardi, F., Gomes, A., Ito, F., Richtzenhain, L., Soares, R., Heinemann, M., Cortez, A., Sakamoto, S., & Maiorka, P. (2005). Biological and immunological studies of five Brazilian rabies virus isolates. Brazilian Journal of Veterinary Research and Animal Science, 42(4), 307-312. https://doi.org/10.11606/issn.1678-4456.bjvras.2005.26426
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