The evaluation of "TF-Test" kit for diagnosis of gastrointestinal parasite infections in sheep

  • Giuliano Lumina Universidade Estadual Paulista, Instituto de Biologia, Departamento de Parasitologia, Botucatu, SP
  • Patrizia Ana Bricarello Centro de Pesquisa em Biotecnologia Orion, São Paulo, SP
  • Jancarlo Ferreira Gomes Centro de Pesquisa em Biotecnologia Orion, São Paulo, SP
  • Alessandro Francisco Talamini do Amarante Universidade Estadual Paulista, Instituto de Biologia, Departamento de Parasitologia, Botucatu, SP
Keywords: Diagnostic, FEC, Haemonchus contortus, Sheep, TF-Test

Abstract

This study was performed to standardize parasite egg counting in feces of sheep by TF-Test, in addition to compare this test to the Gordon & Whitlock technique (G&W). Twenty-four lambs were artificially infected with Haemonchus contortus throughout 12 weeks. At the end of this time, faecal samples were taken and animals were slaughtered for worm identification and counting. G&W and TF-Test methods were carried out on each fecal sample. Both tests showed Haemonchus eggs in 95.8% of the samples (P>;0.05). The correlation coefficients (r) between fecal egg counts (FEC) using G&W x Total Worm Count (TWC) were r=0.52 (not transformed data) and r=0.85 (transformed data); between FEC by TF-Test x TWC were r=0.51 (not transformed data) and r=0.87 (transformed data). Other 100 fecal samples were taken from naturally infected sheep. In these animals, the G&W and TF-Test methods showed 85% and 86% of fecal samples positive for Strongylidea eggs, respectively (P>;0.05). Also in those animals, Eimeria oocysts were found in 33% of fecal samples by TF-Test, whereas in the G&W only 12% were positive (P<0.001). For Strongyloides spp., TF-Test showed 15% of positive fecal samples, whereas G&W showed 5% (P<0.05). In conclusion, both methods were efficient to diagnose gastrointestinal nematodes and TF-Test was superior to diagnose oocysts of Eimeria spp. and eggs of Strongyloides spp; conversely, Strongylidea eggs counting using TF-Test was underestimated.

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Published
2006-08-01
How to Cite
Lumina, G., Bricarello, P., Gomes, J., & Amarante, A. (2006). The evaluation of "TF-Test&quot; kit for diagnosis of gastrointestinal parasite infections in sheep. Brazilian Journal of Veterinary Research and Animal Science, 43(4), 496-501. https://doi.org/10.11606/issn.1678-4456.bjvras.2006.26465
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