Using the REP-PCR technic in the tracking of Staphylococcus aureus in milking room, for milk quality production

  • Lea Chapaval Embrapa Caprinos, Sobral, CE
  • David Henry Moon Universidade de São Paulo, Escola Superior de Agricultura Luiz de Queiroz, Departamento de Genética, Piracicaba, SP
  • José Elias Gomes Universidade de São Paulo, Centro de Energia Nuclear na Agricultura, Piracicaba, SP
  • Fábio Rodrigo Duarte Universidade de São Paulo, Centro de Energia Nuclear na Agricultura, Piracicaba, SP
  • Siu Mui Tsai Universidade de São Paulo, Centro de Energia Nuclear na Agricultura, Piracicaba, SP
Keywords: Staphylococcus aureus, Milking hygiene, Molecular epidemiology, Fingerprint

Abstract

The identification and classification of bacteria are of crucial importance in environmental, industrial, veterinary, microbiology and microbial ecology. A number of different phenotypic and genotypic methods are presently being employed for microbial identification and classification. Repetitive-element PCR (Rep-PCR) with primers based on repetitive extragenic palindromic (REP) repeated DNA sequences is a recently described method which generates DNA fingerprints that descriminate between bacterial species and strains. In this study, this method was used for genomic fingerprinting of Staphylococcus aureus in control of hygiene in milk line production on two farms. Complex fingerprinting patterns were obtained for all isolates from different sources (milking handlers, cows teats, milk and milk machine) were very similar, and the data indicated that the isolated were closely related. In our study, this technic shows to be usefull for investigation in fails on milk line production and for more efficient control for patogenic microrganisms that cause serious illness in humans and animals.

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Published
2006-06-01
How to Cite
Chapaval, L., Moon, D., Gomes, J., Duarte, F., & Tsai, S. (2006). Using the REP-PCR technic in the tracking of Staphylococcus aureus in milking room, for milk quality production. Brazilian Journal of Veterinary Research and Animal Science, 43(3), 309-320. https://doi.org/10.11606/issn.1678-4456.bjvras.2006.26478
Section
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