Microcolony detection of Mycobacterium Bovis in Middlebrook 7H11 thin layer culture

Authors

  • André Guaragna Marcondes Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Deparamento de Medicina Veterinária Preventiva e Saúde Animal, São Paulo, SP
  • Maria de Lourdes M. Shikama Instituto Adolfo Lutz, Laboratório Regional de Sorocaba, Sorocaba, SP
  • Sílvio Arruda Vasconcellos Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Deparamento de Medicina Veterinária Preventiva e Saúde Animal, São Paulo, SP
  • Nílson Roberti Benites Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Deparamento de Medicina Veterinária Preventiva e Saúde Animal, São Paulo, SP
  • Zenaide Maria de Morais Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Deparamento de Medicina Veterinária Preventiva e Saúde Animal, São Paulo, SP
  • Eliana Roxo Instituto Biológico, São Paulo, SP
  • Ricardo Augusto Dias Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Deparamento de Medicina Veterinária Preventiva e Saúde Animal, São Paulo, SP
  • Sylvia Luisa Pincherle Cardoso Leão Universidade Federal de São Paulo, São Paulo, SP
  • Sônia Regina Pinheiro Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Deparamento de Medicina Veterinária Preventiva e Saúde Animal, São Paulo, SP

DOI:

https://doi.org/10.11606/issn.1678-4456.bjvras.2006.26484

Keywords:

Mycobacterium bovis, Cultivation media, Veterinary bacteriology, Middlebrook 7H11

Abstract

The aim of this article is to evaluate the efficiency of the cultivation technique in thin layer of Middlebrook 7H11 (TL7H11) for isolating Mycobactererium bovis from suggestive lesions of tuberculosis in cattle and to compare the results with traditional methods of cultivation. At the first step it was used M. bovis AN5 and M. tubercuolosis H37Rv standard strain. The both performance were compared between the cultivation in TL7H11 and in the Stonebrink and Petragnani media. The strains presented visible growing in TL7H11 at the third day of cultivation, while the Stonebrink and Petragnani there were growing just at the 14 day. At the 13 day of cultivation it was possible to differentiate both strains by their colony morphological characteristics. The second step was to cultivate 62 clinicals samples in TL7H11 and Stonebrink for tentative isolation of M. bovis. The isolated samples were detected in TL7H11 until 21 days of cultivation whereas none samples weregrown in Stonebrink tubes. The median time of growing in TL7H11 was 19,0 days against 49,0 days of Stonebrink (p=0,014).

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Published

2006-06-01

How to Cite

Marcondes, A. G., Shikama, M. de L. M., Vasconcellos, S. A., Benites, N. R., Morais, Z. M. de, Roxo, E., Dias, R. A., Leão, S. L. P. C., & Pinheiro, S. R. (2006). Microcolony detection of Mycobacterium Bovis in Middlebrook 7H11 thin layer culture. Brazilian Journal of Veterinary Research and Animal Science, 43(3), 362-369. https://doi.org/10.11606/issn.1678-4456.bjvras.2006.26484

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