Detection of Leptospira pomona in bovine semen by fluorescent capillary eletrophoresis

Authors

  • Francisca Elda Ferreira Dias Universidade Estadual Paulista, Faculdade de Ciências Agrárias e Veterinárias, Departamento de Medicina Veterinária Preventiva e Reprodução Animal, Jaboticabal, SP
  • Sérgio Morais Aoki Universidade Estadual Paulista, Instituto de Biociências, Departamento de Genética, Botucatu, SP
  • Lígia Garcia Mesquita Universidade Estadual Paulista, Faculdade de Medicina Veterinária, Departamento de Apoio, Produção e Saúde Animal, Araçatuba, SP
  • Caris Maroni Nunes Universidade Estadual Paulista, Faculdade de Medicina Veterinária, Departamento de Apoio, Produção e Saúde Animal, Araçatuba, SP
  • José Fernando Garcia Universidade Estadual Paulista, Faculdade de Medicina Veterinária, Departamento de Apoio, Produção e Saúde Animal, Araçatuba, SP

DOI:

https://doi.org/10.11606/issn.1678-4456.bjvras.2006.26488

Keywords:

Semen Bovine, Leptospira, PCR, Capillary Eletrophoresis

Abstract

This study was performed in order to evaluate the detection limit of PCR with fluorescent capillary electrophoresis for Leptospira pomona diagnosis in bovine semen. Negative bovine semen samples were artificially contaminated with Leptospira pomona (10(0) to 10(7) bacteria/ml) and DNA was extracted by phenol/chloroform protocol. DNA fragments visualization was done by three electrophoresis methods: under UV light in 2% agarose gel, silver staining 8% polyacrylamide gel and fluorescent capillary electrophoresis. The detection limit of capillary electrophoresis for Leptospira pomona was 10²bacteria/ml. Under UV light, in 2% agarose gel, the detection limit was of 10(4) bacteria/ml while for silver stained 8% polyacrylamide gel it was 10² bacteria/ml. PCR with fluorescent capillary electrophoresis is an efficient and rapid diagnostic test for DNA detection of Leptospira in bovine semen and this can be an important tool for herd and semen sanitary control in artificial insemination centers.

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Published

2006-06-01

How to Cite

Dias, F. E. F., Aoki, S. M., Mesquita, L. G., Nunes, C. M., & Garcia, J. F. (2006). Detection of Leptospira pomona in bovine semen by fluorescent capillary eletrophoresis. Brazilian Journal of Veterinary Research and Animal Science, 43(3), 394-399. https://doi.org/10.11606/issn.1678-4456.bjvras.2006.26488

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