Experimental chronic granulomatous inflammatory process in fish: a morphological, ultrastructural and immunocytochemical study

  • Eliana Reiko Matushima Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Patologia, São Paulo, SP
  • Adhemar Longatto Filho Instituto Adolfo Lutz, Divisão de Patologia, São Paulo, SP
  • Cristina Takami Kanamura Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Patologia, São Paulo, SP
  • Idércio Luiz Sinhorini Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Patologia, São Paulo, SP
Keywords: Inflammation, Fish, Nile tilapia

Abstract

The purpose of the present study was to evaluate the chronic granulomatous inflammatory process experimentally induced in Oreochromis niloticus by the inoculation of BCG and to clarify aspects of the inflammatory reaction in fish for a better understanding of the phylogeny of the process. Results obtained by light microscopy and ultrastructural analysis showed the participation of macrophages, thrombocytes, lymphocytes, eosinophils, plasma cells and foreign type giant cells in the inflammatory process. Besides these cell types, a granulomatous reaction mainly consisting of epithelioid cells was also observed ultrastructurally. These epithelioid cells developed desmosomes throughout the experiment, and also began to express receptors for cytokeratin. Both findings are immunocytochemically characteristic of epithelial cells. Pigment cells (melanomacrophages) could also be seen surrounding all the granulomatoid formation in a crescent fashion and participating in the chronic granulomatous inflammatory reaction.

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Published
2006-04-01
How to Cite
Matushima, E., Longatto Filho, A., Kanamura, C., & Sinhorini, I. (2006). Experimental chronic granulomatous inflammatory process in fish: a morphological, ultrastructural and immunocytochemical study. Brazilian Journal of Veterinary Research and Animal Science, 43(2), 152-158. https://doi.org/10.11606/issn.1678-4456.bjvras.2006.26494
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