Antibiogram as instrument of phenotypic typing of Staphylococcus aureus isolated from personnel, raw milk and minas frescal cheese in a dairy plant of Goiás, Brazil

Authors

  • Maria Cláudia Dantas Porfirio Borges André Universidade Federal de Goiás, Instituto de Patologia Tropical e Saúde Pública, Goiânia, GO
  • Patrícia Pimentel Santos Universidade Federal de Goiás, Instituto de Patologia Tropical e Saúde Pública, Goiânia, GO
  • Maria Raquel Hidalgo Campos Universidade Federal de Goiás, Faculdade de Nutrição, Goiânia, GO
  • Liana Jayme Borges Universidade Federal de Goiás, Faculdade de Nutrição, Goiânia, GO
  • Álvaro Bisol Serafini Universidade Federal de Goiás, Instituto de Patologia Tropical e Saúde Pública, Goiânia, GO

DOI:

https://doi.org/10.11606/issn.1678-4456.bjvras.2006.26542

Keywords:

Cheese, Personnel, Antibiotic susceptibility, Staphylococcus aureus

Abstract

During a year (March/2004 to February/2005) 140 samples of raw milk, dairy staff, and Minas Frescal cheese were analyzed for the presence of S. aureus in a dairy plant in Goiás, Brazil. Strains were isolated from 75% of the personnel investigated resulting in 31 (33.7%) isolates from 92 collected samples (46 from hands and 46 from nasopharynx). The 24 raw milk samples resulted in 18 (75.0%) positive samples and 26 isolates, with mean number: 1.1 x 10(5) CFU/ml. Among 24 cheese samples 17 (70.8%) were positive resulting in 20 isolates, with mean number: 3.8 x 104 CFU/g. Thirteen samples (54.2%) were above the limit established by the brazilian legislation (10³ CFU/g). The differentiation of strains was performed by antimicrobial susceptibility test. All isolates were susceptible to ciprofloxacin and gentamicin. Resistance was observed in five (6.5%) isolates for erythromycin, 19 (24.7%) for tetracycline, one (1.3%) for vancomycin, four (5.2%) for oxacillin and 53 (68.8%) isolates for penicillin. Eleven isolates (14.3%) were susceptible to all antibiotics tested and 18 (23.4%) were resistant to more than one antibiotic. The antibiotic susceptibility allowed the discrimination of isolates in 12 different profiles (A-L), but the phenotypic typing failed to determine the source of bacteria for the cheese.

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Published

2006-12-03

How to Cite

André, M. C. D. P. B., Santos, P. P., Campos, M. R. H., Borges, L. J., & Serafini, Álvaro B. (2006). Antibiogram as instrument of phenotypic typing of Staphylococcus aureus isolated from personnel, raw milk and minas frescal cheese in a dairy plant of Goiás, Brazil. Brazilian Journal of Veterinary Research and Animal Science, 43(supl.), 102-108. https://doi.org/10.11606/issn.1678-4456.bjvras.2006.26542

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