Morphologic and morphometry of rectus abdominis muscle fiber types of the during the gestation dog (Canis familiaris)

  • Maila Karina Mattos de Brito Universidade Estadual Paulista, Faculdade de Ciências e Tecnologia, Presidente Prudente, SP
  • Colombo Guerra Carvalho Junior Universidade do Oeste Paulista, Presidente Prudente, SP
  • Alexandre Dal Pai Faculdade de Tecnologia, Botucatu, SP
  • Vitalino Dal Pai Universidade do Oeste Paulista, Presidente Prudente, SP
  • José Alberto Giacometti Universidade Estadual Paulista, Faculdade de Ciências e Tecnologia, Presidente Prudente, SP
Keywords: Retus abdominis muscle, Morphometry, Fiber type I and II, Gestation, Female dog

Abstract

The present paper deals with the evaluation of morphological and morphometric alterations of slow-twist (I) and fast-twitch (II) fibers of rectus abdominis muscle of adult female dog during pre-gestational phase, at 30 and 60 days of pregnancy and at 30 days after the parturition. At the every phases, using the open biopsy technique, muscle samples were collected. The samples were plunged. Histological sections were cut in a microtome. For general morphology, some sections were stained with HE. Subsequent sections were reacted for myofibrillar ATPase (m-ATPase), after alkaline (pH 10.4) and acid (pH 4.4) pre-incubations, in order to identificate type I and II fibers. In the pre-gestational phase, muscle tissue revealed to be composed by fibers with different diameters, presenting polygonal outlines and one or more periphery nuclei. At 30 days of pregnancy, muscle fiber characteristics were similar. At 60 days, in addition to the existence of normal fibers, polymorphic and small diameter fibers were frequent. At 30 days after the parturation, the morphology of muscle fiber were similar to that observed in the pre-gestational phase. In the four phases, type II fiber diameters were lager than type I. The diameters of both fiber types showed a significant reduction in the 30 days phase and a significative increasing at 60 days. The expansion of the abdominal wall during the pregnancy represents a chronic stimulus, induced changes in the morphology and in the fiber type diameters.

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Published
2007-08-01
How to Cite
Brito, M., Carvalho Junior, C., Dal Pai, A., Dal Pai, V., & Giacometti, J. (2007). Morphologic and morphometry of rectus abdominis muscle fiber types of the during the gestation dog (Canis familiaris). Brazilian Journal of Veterinary Research and Animal Science, 44(4), 254-260. https://doi.org/10.11606/issn.1678-4456.bjvras.2007.26625
Section
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