Comparative studies of three methods for detection Theileria equi antibody in endemic areas horses from Rio de Janeiro state

  • Tiago Marques dos Santos Universidade Federal Rural do Rio de Janeiro, Instituto de Veterinária, Seropédica, RJ
  • Priscila Nogueira Ferraz Ministério da Agricultura, Pecuária e Abastecimento, Novo Mundo, MS
  • Fernando Queiroz de Almeida Universidade Federal Rural do Rio de Janeiro, Instituto de Veterinária, Departamento de Medicina e Cirurgia Veterinária, Seropédica, RJ
  • Carlos Luiz Massard Universidade Federal Rural do Rio de Janeiro, Instituto de Veterinária, Departamento de Parasitologia Animal, Seropédica, RJ
  • Cristiane Divan Baldani Universidade Federal do Tocantins, Escola de Medicina Veterinária e Zootecnia, Aragauaina, TO
  • Paulo de Tarso Landgraf Botteon Universidade Federal Rural do Rio de Janeiro, Instituto de Veterinária, Departamento de Medicina e Cirurgia Veterinária, Seropédica, RJ
  • Huarrisson Azevedo Santos Universidade Federal Rural do Rio de Janeiro, Instituto de Veterinária, Seropédica, RJ
  • Rosangela Zacarias Machado Universidade Estadual Paulista, Faculdade de Ciências Agrárias e Veterinárias, Departamento de Patologia Veterinária, Jaboticabal, SP
  • Cláudio de Moraes Andrade Empresa de Pesquisa Agropecuária do Estado do Rio e Janeiro, Niterói, RJ
Keywords: Babesiosis, Equine, Theileria equi, Serological diagnostic

Abstract

This study was carried out to evaluate indirect fluorescent antibody test (IFAT), enzyme-linked immunosorbent assay (ELISA) and complement fixation test (CFT) of Theileria equi diagnosis in sera samples of 79 horses at Universidade Federal Rural do Rio de Janeiro (UFRRJ), Seropédica, RJ, Brazil were tested. Positive reaction was obtained in 74.7, 75.9 and 60.8% of samples tested by IFAT, indirect ELISA and CFT, respectively. Discrepancy was observed in 16.45% (n=13) of serum samples tested by ELISA and IFAT. While IFAT and CFT were compared, the discrepancy observed among the samples tested were 36.71% (n=29). Indirect ELISA and CFT test presented disagreement in 37.97% (n=30) of serum samples tested. Results of present study suggests that the best alternative for serological diagnosis T. equi in carriers horses is the combined use of IFAT and indirect ELISA test, especially for accomplishment of seroepidemiological studies.

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Published
2009-12-01
How to Cite
Santos, T., Ferraz, P., Almeida, F., Massard, C., Baldani, C., Botteon, P., Santos, H., Machado, R., & Andrade, C. (2009). Comparative studies of three methods for detection Theileria equi antibody in endemic areas horses from Rio de Janeiro state. Brazilian Journal of Veterinary Research and Animal Science, 46(6), 484-490. https://doi.org/10.11606/S1413-95962009000600007
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