Development of the leptospirosis by experimental infection in hamsters (Mesocricetus auratus) with Leptospira interrogans serovar Canicola, strain LO4, by intact and scratched skin exposures

  • Carolina de Sousa Américo Batista Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Medicina Veterinária Preventiva e Saúde Animal, São Paulo, SP
  • Sérgio Santos de Azevedo Universidade Federal de Campina Grande, Centro de Saúde e Tecnologia Rural, Unidade Acadêmica de Medicina Veterinária, Patos, PB
  • Sílvio Arruda Vasconcellos Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Medicina Veterinária Preventiva e Saúde Animal, São Paulo, SP
  • Vanessa Castro Instituto Biológico, Centro de Pesquisa e Desenvolvimento em Sanidade Animal, São Paulo, SP
  • Salomão Moreira de Figueiredo Universidade Federal de Campina Grande, Centro de Saúde e Tecnologia Rural, Unidade Acadêmica de Medicina Veterinária, Patos, PB
  • Clebert José Alves Universidade Federal de Campina Grande, Centro de Saúde e Tecnologia Rural, Unidade Acadêmica de Medicina Veterinária, Patos, PB
  • Flávia Carolina Souza de Oliveira Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Medicina Veterinária Preventiva e Saúde Animal, São Paulo, SP
  • Margareth Élide Genovez Instituto Biológico, Centro de Pesquisa e Desenvolvimento em Sanidade Animal, São Paulo, SP
Keywords: Leptospiorosis, Experimental infection, Hamster, Skin exposure, Serovar Canicola (LO4 strain)

Abstract

The establishment and evolution of leptospirosis in hamster (Mesocricetus auratus) by experimental infection with Leptospira interrogans serovar Canicola, LO4 strain, by intact and scratched skin exposures, having as control the intraperitoneal route, were evaluated. Hundred-twenty female hamsters distributed in two groups according to inoculation route (intact and scratched skin) were used. Infectious inoculum was constituted by a pure culture of L. interrogans serovar Canicola (strain LO4), isolated from liver from a slaughtered swine in Londrina, Paraná state and typified by agglutinins adsortion technique with monoclonal antibody kit at the Royal Tropical Institute, Amsterdam, the Netherlands. The animals were observed twice a day during 21 days. Animals that died were necropsied and kidneys, liver, genital tract (uterus and ovaries) and brain were aseptically collected. On the 21st post-inoculation day, surviving animals were euthanized. In these animals, serum samples were also collected by cardiac puncture to antileptospires agglutinins research using microscopic agglutination test (MAT). Fresh direct microscopy and microbiological culture were used for the detection of leptospires. Scratched skin route induced larger lethality when compared to intact skin route, with establishment and evolution of leptospirosis. On the other hand, intact skin route induced renal and/or genital carrier state more frequently. LO4 strain presented low immunogenic power, characterized by soroconversion at the MAT in only one inoculated animal.

Downloads

Download data is not yet available.
Published
2010-10-01
How to Cite
Batista, C., Azevedo, S., Vasconcellos, S., Castro, V., Figueiredo, S., Alves, C., Oliveira, F., & Genovez, M. (2010). Development of the leptospirosis by experimental infection in hamsters (Mesocricetus auratus) with Leptospira interrogans serovar Canicola, strain LO4, by intact and scratched skin exposures. Brazilian Journal of Veterinary Research and Animal Science, 47(5), 346-351. https://doi.org/10.11606/issn.1678-4456.bjvras.2010.26814
Section
UNDEFINIED