Immunological and genetic study of 10 bat rabies virus isolates from Rio de Janeiro State, Southeast Brazil

  • Carla da Silva Mota Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Medicina Veterinária Preventiva e Saúde Animal, São Paulo, SP
  • Fumio Honma Ito Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Medicina Veterinária Preventiva e Saúde Animal, São Paulo, SP
  • Marlon Vicente Silva Instituto Municipal de Medicina Veterinária Jorge Vaitsman, Laboratório de Diagnóstico da Raiva, Rio de Janeiro, RJ
  • Go Sato College of Bioresource Sciences, Department of Preventive Veterinary Medicine and Animal Health, Veterinary Research Center, Tóquio
  • Yuki Kobayashi College of Bioresource Sciences, Department of Preventive Veterinary Medicine and Animal Health, Veterinary Research Center, Tóquio
  • Takuya Itou College of Bioresource Sciences, Department of Preventive Veterinary Medicine and Animal Health, Veterinary Research Center, Tóquio
  • Takeo Sakai College of Bioresource Sciences, Department of Preventive Veterinary Medicine and Animal Health, Veterinary Research Center, Tóquio
Keywords: Rabies virus, Rabies vaccine, Vaccine Potency, Bats, Phylogeny

Abstract

In the present study we analyzed ten bats rabies viruses isolated from Rio de Janeiro State, focusing on its genetic characteristics from genes N and G, and also in the response of mice vaccinated with cell-culture rabies vaccine, produced with the Pitman-Moore strain, after viral challenge with bat rabies isolates, using an immunologic essay based on NIH vaccine potency test. The vaccine used conferred protection in more than 80% of the mice vaccinated with 1:15 vaccine dilution, after viral challenge. N gene genetic analysis divided the rabies virus isolates into haematophagous and insectivorous bat variants, with all isolates from Artibeus sp. frugivorous bats being clustered with the variant characteristic of the Desmodus rotundus vampire bat. Phylogenetic differences between isolates from Northeast Region and those from the Metropolitan and South Regions of Rio de Janeiro State were observed. The substitution of an aspartic acid to a glutamic acid found in the position 118 of G gene genetic characterization from samples 704/97BR-DR and 151/98BR-DR seems to be related to viral antigenicity. There were no time-related genetic differences between the studied samples. The vaccine employed was found with satisfactory protection against the majority of the isolates used.

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Published
2010-02-01
How to Cite
Mota, C., Ito, F., Silva, M., Sato, G., Kobayashi, Y., Itou, T., & Sakai, T. (2010). Immunological and genetic study of 10 bat rabies virus isolates from Rio de Janeiro State, Southeast Brazil. Brazilian Journal of Veterinary Research and Animal Science, 47(1), 74-85. https://doi.org/10.11606/issn.1678-4456.bjvras.2010.26851
Section
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