In vitro maturation and embryo development of bovine oocytes after meiosis blockage with MPF inhibitors

  • Mariana Groke Marques Empresa Brasileira de Pesquisa Agropecuária, Centro Nacional de Pesquisa de Suínos e Aves, Concórdia, SC
  • Marco Roberto Bourg de Mello Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ
  • Liliam Mara Trevisan Tavares Universidade Estadual do Ceará, Faculdade de Educação Ciências e Letras do Sertão Central, Quixadá, CE
  • Alessandra Corallo Nicacio Universidade Estadual do Maranhão, São Luís, MA
  • Mayra Elena Ortiz D'Avila Assumpção Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Reprodução Animal, São Paulo, SP
  • José Antonio Visintin Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Reprodução Animal, São Paulo, SP
Keywords: Oocyte maturation, Butyroloactone I, Roscovitine, In vitro embryo production

Abstract

This study evaluated the bovine oocyte maturation and embryo development after in vitro fertilization. The maturation of the oocytes was blocked using Butyrolactone I and Roscovitine using pre-maturation medium supplemented with fetal calf serum (FCS). The ocytes were divided in four groups: Control 0 hour, Control (24 hours of maturation), Roscovitine (maturation blockage with 50mM Roscovitine during 24 hours followed by 24 hours of maturation), and Butyrolactone I (maturation blockage with 150mM Butyrolactone I during 24 hours followed by 24 hours of maturation). The oocytes were fixed and stained with aceto orcein to evaluate the nuclear maturation. After the maturation period, the remaining oocytes of the Control group, Roscovitine, and Butyrolactone I were fertilized in vitro. Embryo development was assessed by the cleavage rate (D3) and blastocysts formation (D7). The Butyrolactone I group had similar rates of germinal vesical stage oocytes during blockage, and Metaphase 2 after maturation, comparing to Control group at 0 hour and Control group, respectively. On the other hand, the Roscovitine group had lower rates of vesical stage oocytes during blockage, and Metaphase 2 after maturation comparing to Control groups. After in vitro fertilization, higher rates of cleavage were observed in Control and Butyrolactone I groups. For the blastocyst formation rate, the Control group showed better results than Roscovitine group. In summary, Butyrolactone I group had similar results to the Control group, and for this reason, is suitable for pre-maturation of bovine oocytes using FCS. In contrast, Roscovitine group had lower oocyte maturation and embryo development.

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Published
2011-12-01
How to Cite
Marques, M., Mello, M., Tavares, L., Nicacio, A., Assumpção, M., & Visintin, J. (2011). In vitro maturation and embryo development of bovine oocytes after meiosis blockage with MPF inhibitors. Brazilian Journal of Veterinary Research and Animal Science, 48(6), 468-477. https://doi.org/10.11606/S1413-95962011000600005
Section
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