Influence of different forms of culture on the nitric oxide action on maturation and membrane integrity on bovine cumulus-oocyte complex

  • Kelen Salaroli Viana Universidade Estadual do Norte Fluminense, Centro de Ciências e Tecnologias Agropecuárias, Laboratório de Reprodução e Melhoramento Genético Animal, Campos dos Goytacazes, RJ
  • Maria Clara Caldas-Bussiere Universidade Estadual do Norte Fluminense, Centro de Ciências e Tecnologias Agropecuárias, Laboratório de Reprodução e Melhoramento Genético Animal, Campos dos Goytacazes, RJ
  • Carla Sobrinho Paes de Carvalho Universidade Estadual do Norte Fluminense, Centro de Ciências e Tecnologias Agropecuárias, Laboratório de Reprodução e Melhoramento Genético Animal, Campos dos Goytacazes, RJ
  • Bruna Lomba Dias Universidade Estadual do Norte Fluminense, Centro de Ciências e Tecnologias Agropecuárias, Laboratório de Reprodução e Melhoramento Genético Animal, Campos dos Goytacazes, RJ
  • Verônica R. Lanes Universidade Estadual do Norte Fluminense, Centro de Biociências e Biotecnologia, Laboratório de Biologia do Reconhecer, Campos dos Goytacazes, RJ
  • Celia Raquel Quirino Universidade Estadual do Norte Fluminense, Centro de Ciências e Tecnologias Agropecuárias, Laboratório de Reprodução e Melhoramento Genético Animal, Campos dos Goytacazes, RJ
Keywords: Nitric oxide, Mineral oil, In vitro maturation, Sodium nitroprusside, In vitro culture, Bovine

Abstract

The aim of the present study was to evaluate the influence of different forms of in vitro culture on the nitric oxide action in maturation and membrane integrity on bovine cumulus-oocyte complex. No significant effect was observed between different forms of culture (mineral oil vs plate; P > 0.05), as much for membrane integrity as for expansion of the CC. However, it was observed that oocytes of the groups control and 10-3 M of SNP, cultivated in plate, had presented greater percentage of cell with maintenance of membrane integrity than same treatments cultivated in drop. The addition of 10-3 M of SNP showed an inhibitory effect on the expansion and membrane integrity of CC and oocytes in both, culture in drops under oil and plate (P < 0.05). The culture form did not intervene with the extrusion of the first polar corpuscle and the addition of 10-3 M of SNP inhibited this extrusion in the both systems (P < 0.05). There was a dose-response effect on the concentration of NO in the maturation medium in both types of cultivation (P < 0,05), and this was higher in the culture medium under oil, except when added 10-3 M of SNP, treatment in which there was no difference in the types of cultivation employed. (P<0.05). These data demonstrate that the culture system did not intervene with the action of the NO in the maturation in vitro of bovine COC, but intervened with the integrity of the plasmatic membrane of the oocyte.

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Published
2011-04-01
How to Cite
Viana, K., Caldas-Bussiere, M., Carvalho, C., Dias, B., Lanes, V., & Quirino, C. (2011). Influence of different forms of culture on the nitric oxide action on maturation and membrane integrity on bovine cumulus-oocyte complex. Brazilian Journal of Veterinary Research and Animal Science, 48(2), 147-154. https://doi.org/10.11606/S1413-95962011000200007
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