Total fraction of mononuclear cell or stromal vascular fraction transplant associated with cellulosic membrane in experimental cutaneous wounds in rabbits

Authors

  • Tiago Luis Eilers Treichel Universidade Federal de Santa Maria, Santa Maria, RS
  • Marina Gabriela Monteiro Carvalho Mori da Cunha Universidade Federal de Santa Maria, Santa Maria, RS
  • João Paulo Monteiro Carvalho Mori da Cunha Universidade Federal de Santa Maria, Santa Maria, RS
  • Eduardo de Bastos Santos Júnior Universidade Federal de Santa Maria, Santa Maria, RS
  • Paulo de Tarso de Oliveira Leme Júnior Universidade Federal de Santa Maria, Santa Maria, RS
  • Márcio Machado Costa Universidade Federal de Santa Maria, Santa Maria, RS
  • Ney Luis Pippi Universidade Federal de Santa Maria, Departamento de Clínica de Pequenos Animais, Santa Maria, RS
  • Sônia Teresinha dos Anjos Lopes Universidade Federal de Santa Maria, Departamento de Clínica de Pequenos Animais, Santa Maria, RS
  • Rafael Almeida Fighera Universidade Federal de Santa Maria, Departamento de Patologia, Santa Maria, RS
  • Maurício Borges da Rosa Universidade Federal de Santa Maria, Santa Maria, RS

DOI:

https://doi.org/10.11606/S1413-95962011000100008

Keywords:

Fraction of cells, Repair, Wound, Skin, Rabbit

Abstract

The skin presents various important functions to the organism. The maintenance of its integrity is fundamental, among others, to prevent penetration of microorganisms and exit of liquids essential to life maintenance. Due to its constant environment exposure, the skin is highly susceptible to trauma which can result solutions of continuity. The healing of wounded skin should be fast and many alternatives are searched for, aiming to reduce repair time and to guarantee a functional and esthetically acceptable scar. One option to repair injured tissues which is ever more viable is cellular therapy with adult stem cells. The aims of this study consisted in evaluating the transplant of total mononuclear cell fraction (TMCF) from bone marrow (BM) or stromal vascular fraction (SVF) from adipose tissue (AT), associated with the use of cellulose membrane. To carry out this experiment, 20 rabbits were randomly divided in four groups with five animals each. After induction of the experimental cutaneous wound, Group A did not receive any treatment; group B received only cellulose membrane; and groups C and D, in addition to receiving the membrane, were submitted to autologous transplant of TMCF, with total cell value between 6,92 x 10(6) and 4,91 x 10(7) and a viability of 82 to 97% or SVF, with total cell value between 9,6 x 10(5) and 6,5 x 10(6) and a viability of 66 a 87%, respectively. At the end of the evaluation period, the three treated groups presented significant statistical difference of wound area in relation to the control group, and the group which received SVF from adipose tissue presented the shortest wound healing time.

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Published

2011-02-01

How to Cite

Treichel, T. L. E., Cunha, M. G. M. C. M. da, Cunha, J. P. M. C. M. da, Santos Júnior, E. de B., Leme Júnior, P. de T. de O., Costa, M. M., Pippi, N. L., Lopes, S. T. dos A., Fighera, R. A., & Rosa, M. B. da. (2011). Total fraction of mononuclear cell or stromal vascular fraction transplant associated with cellulosic membrane in experimental cutaneous wounds in rabbits. Brazilian Journal of Veterinary Research and Animal Science, 48(1), 62-72. https://doi.org/10.11606/S1413-95962011000100008

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