Influence of sire breed (Bos indicus vs Bos taurus) on heat stress tolerance of in vitro-produced bovine embryos

  • Thaís Nabhan Universidade Estadual Paulista, Instituto de Biociências, Departamento de Farmacologia, Botucatu, SP
  • Rafael Augusto Satrapa Universidade Estadual Paulista, Instituto de Biociências, Departamento de Farmacologia, Botucatu, SP
  • Renato Arantes Lima Simões Universidade Estadual Paulista, Instituto de Biociências, Departamento de Farmacologia, Botucatu, SP
  • Cíntia Fernandes da Silva Universidade Estadual Paulista, Instituto de Biociências, Departamento de Farmacologia, Botucatu, SP
  • Eduardo Montanari Razza Universidade Estadual Paulista, Instituto de Biociências, Departamento de Farmacologia, Botucatu, SP
  • Raquel Puelker Universidade Estadual Paulista, Instituto de Biociências, Departamento de Farmacologia, Botucatu, SP
  • Luzia Aparecida Trinca Universidade Estadual Paulista, Instituto de Biociências, Departamento de Bioestatística, Botucatu, SP
  • Ciro Moraes Barros Universidade Estadual Paulista, Instituto de Biociências, Departamento de Farmacologia, Botucatu, SP
Keywords: Blastocyst, Bovine, In vitro production, Heat stress

Abstract

To better understand the differences related to HS resistance between Bos indicus and Bos taurus, we aim to verify if the HS tolerance is due mostly to the genetic contribution from the oocyte, spermatozoa or both. Oocytes from Nelore and crossbreed Holstein cows (cHST) were collected, matured and fertilized with semen from Nelore (N), Angus (An), Brahman (Bra) and Gir (Gir) bulls. Nine six hours post insemination (hpi), > 16 cells embryos were separated in two groups: control and HS. In control group, embryos were cultured at 39 ºC, whereas in the HS group, embryos were subjected to 41 ºC for 12 h, and then returned to 39 ºC. There was no effect of HS on blastocyst and hatched blastocyst rates in all breeds analyzed. The percentage of oocytes that cleaved and reached morula stage was significantly lower (p < 0.05) in cHST x Gir as compared to the other breeds. Additionally, blastocyst rates was higher in cHST x N than in cHST x An and cHST x Gir (p < 0.05). It was concluded that the oocyte is more important than the spermatozoa for the development of thermotolerance, since the breed of the bull did not influence embryo development after HS.

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Published
2011-08-01
How to Cite
Nabhan, T., Satrapa, R., Simões, R., Silva, C., Razza, E., Puelker, R., Trinca, L., & Barros, C. (2011). Influence of sire breed (Bos indicus vs Bos taurus) on heat stress tolerance of in vitro-produced bovine embryos. Brazilian Journal of Veterinary Research and Animal Science, 48(4), 336-343. https://doi.org/10.11606/S1413-95962011000400010
Section
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