Semen evaluation of captive male red-winged tinamous (Rhynchotus rufescens) supplemented with selenium

  • Paola Almeida de Araújo Góes Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Reprodução Animal, São Paulo, SP
  • Ana Karina da Silva Cavalcante Universidade Metropolitana de Salvador, Departamento de Reprodução, Salvador, BA
  • Aline Frasseto Tavian Universidade Estadual Paulista, Faculdade de Ciências Agrárias e Veterinárias, Departamento de Zootecnia, Jaboticabal, SP
  • Leticia Felipe Universidade Metropolitana de Salvador, Departamento de Reprodução, Salvador, BA
  • Erika Coelho Santos Universidade Estadual Paulista, Faculdade de Ciências Agrárias e Veterinárias, Departamento de Zootecnia, Jaboticabal, SP
  • Marcílio Nichi Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Reprodução Animal, São Paulo, SP
  • Sandra Aidar de Queiroz Universidade Estadual Paulista, Faculdade de Ciências Agrárias e Veterinárias, Departamento de Zootecnia, Jaboticabal, SP
  • Renato Campanarut Barnabe Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Reprodução Animal, São Paulo, SP
  • Valquíria Hyppolito Barnabe Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Reprodução Animal, São Paulo, SP
Keywords: Bird semen collection, Selenium, Red-winged tinamou

Abstract

Due to the commercial importance of the red-winged tinamou (Rhynchotus rufescens), for the past few years, the employment of reproductive biotechnologies has been attempted. Thirty animals were randomly assigned into two groups: control group (no selenium) and treatment group (supplemented with 0,2 a 0,8 mg selenium/ 1000 kg ration). Animals were allocated at the FCAV - UNESP/Jaboticabal (2007-2008). Semen collections were performed by digital manipulation and divided in pools of at least 150 µL. After the immediate evaluation of motility, vigour, concentration and morphology, an aliquot of 20 µL was diluted in 300 µL of physiologic solution in order to test acrosome and membrane integrities, which were performed by counting 200 cells for each test. Cells were evaluated as follows: 1) Intact acrosome: lilac acrosome; Non-intact acrosome: pink acrosome; 2) Live cells: non stained; Dead: stained. Data was statistically analysed using the SAS System for Windows. No differences were found between treatment and control groups for volume, motility, vigour, mean number of spermatozoa per animal, concentration, Intact acrosome, Intact membrane. The difference found on midpiece sperm defect (Se = 1,33 ± 0,53 and control = 3,78 ± 0,69, p = 0.0107) may be due to the damages caused by the selenium deficiency to the architecture of the midpiece, which compromises sperm mobility and fertilization capacity.

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Published
2011-10-01
How to Cite
Góes, P., Cavalcante, A., Tavian, A., Felipe, L., Santos, E., Nichi, M., Queiroz, S., Barnabe, R., & Barnabe, V. (2011). Semen evaluation of captive male red-winged tinamous (Rhynchotus rufescens) supplemented with selenium. Brazilian Journal of Veterinary Research and Animal Science, 48(5), 370-377. https://doi.org/10.11606/S1413-95962011000500003
Section
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