Detection of infectious bronchitis virus and Avian metapneumovirus by a duplex semi-nested reverse transcription–polymerase chain reaction assay

  • Ana Maria Acevedo Department of Animal Virology, Microbiology Direction. National Center for Animal and Plant Health, Havana, Cuba; Coronavirus Research Group, School of Veterinary Medicine, University of São Paulo
  • Laura Yaneth B. Villarreal Coronavirus Research Group, School of Veterinary Medicine, University of São Paulo
  • Leonardo José Richtzenhain Department of Preventive Veterinary Medicine and Animal Health, School of Veterinary Medicine, University of São Paulo; Coronavirus Research Group, School of Veterinary Medicine, University of São Paulo
  • Julia Noda Department of Animal Virology, Microbiology Direction. National Center for Animal and Plant Health, Havana
  • Paulo Eduardo Brandão Department of Preventive Veterinary Medicine and Animal Health, School of Veterinary Medicine, University of São Paulo; Coronavirus Research Group, School of Veterinary Medicine, University of São Paulo
Keywords: IBV. aMPV. PCR. Diagnosis.

Abstract

A duplex RT-PCR assay is reported for the simultaneous detection of avian infectious bronchitis virus (IBV) and avian metapneumovirus (aMPV), the causative agents of major diseases in poultry. The duplex RT-PCR assay optimized showed a detection limit of 10-3 (101 EID50/50m L for IBV and 100.5 EID50/50m L for aMPV, respectively when two viruses were mixed and 10-1 for each one separated (103 EID50/50m L for IBV and 102.5 EID50/50m L for aMPV, respectively. It was specific, sensitive and applicable for the rapid detection of these viruses in clinical samples.

 

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Published
2012-06-06
How to Cite
Acevedo, A. M., Villarreal, L., Richtzenhain, L., Noda, J., & Brandão, P. (2012). Detection of infectious bronchitis virus and Avian metapneumovirus by a duplex semi-nested reverse transcription–polymerase chain reaction assay. Brazilian Journal of Veterinary Research and Animal Science, 49(3), 221-224. https://doi.org/10.11606/issn.1678-4456.v49i3p221-224
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