Use of DNA markers for genomic diagnosis of domestic animals: 1. Detection of point mutation causing Bovine Leukocyte Adhesion Deficiency (BLAD) in Brazilian Holstein cattle

  • José Fernando Garcia Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, São Paulo, SP
  • André Sanches do Amaral Gurgel Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, São Paulo, SP
  • José Antonio Visintin Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, São Paulo, SP
  • Vagner Ricardo Lunge Universidade Federal do Rio Grande do Sul, Centro de Biotecnologia do Estado do Rio Grande do Sul, Porto Alegre, RS
  • Isidro Hoetzel Universidade Federal do Rio Grande do Sul, Centro de Biotecnologia do Estado do Rio Grande do Sul, Porto Alegre, RS
Keywords: Leukocytes, Bovine, DNA.

Abstract

Ten Holstein bovines, descendants from Ivanhoe line, have been analyzed. DNAs, purified from leukocytes of those animals, were submitted to Polymerase Chain Reaction technique (PCR) and digested by Hae III and Taq I restriction enzymes. Accordingly, the bovine leukocyte adhesion deficiency (BLAD) genomic diagnosis was established. The tests revealed that 2 animals were carriers and 8 were normal. Since the development of PCR methodology, a fast, practical and efficient way to select bulls in Artificial Insemination Centers has been through detection of carrier and normal animals.

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Published
1996-09-01
How to Cite
Garcia, J. F., Gurgel, A., Visintin, J. A., Lunge, V. R., & Hoetzel, I. (1996). Use of DNA markers for genomic diagnosis of domestic animals: 1. Detection of point mutation causing Bovine Leukocyte Adhesion Deficiency (BLAD) in Brazilian Holstein cattle. Brazilian Journal of Veterinary Research and Animal Science, 33(3), 133-135. https://doi.org/10.11606/issn.2318-3659.v33i3p133-135
Section
BASIC SCIENCES