Use of DNA markers for genomic diagnosis of domestic animals: 1. Detection of point mutation causing Bovine Leukocyte Adhesion Deficiency (BLAD) in Brazilian Holstein cattle
AbstractTen Holstein bovines, descendants from Ivanhoe line, have been analyzed. DNAs, purified from leukocytes of those animals, were submitted to Polymerase Chain Reaction technique (PCR) and digested by Hae III and Taq I restriction enzymes. Accordingly, the bovine leukocyte adhesion deficiency (BLAD) genomic diagnosis was established. The tests revealed that 2 animals were carriers and 8 were normal. Since the development of PCR methodology, a fast, practical and efficient way to select bulls in Artificial Insemination Centers has been through detection of carrier and normal animals.
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