Use of DNA markers for genomic diagnosis of domestic animals: 2. Detection of point mutation causing Hyperkalemic Periodic Paralysis (HYPP) in Quarter horses

  • José Fernando Garcia Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, São Paulo, SP
  • André Sanches do Amaral Gurgel Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, São Paulo, SP
  • José Antonio Visintin Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, São Paulo, SP
  • Vagner Ricardo Lunge Universidade Federal do Rio Grande do Sul, Centro de Biotecnologia do Estado do Rio Grande do Sul, Porto Alegre, RS
  • Marcelo Barbante Duarte Rancho’s Tânia, Uberaba, MG
  • Jarbas Leonel Bertolli Associação Brasileira dos Criadores de Cavalo Quarto de Milha, São Paulo, SP
Keywords: Paralysis, Hyperkalemia, Horses, Equidae, DNA.

Abstract

Twelve Quarter horses, descendants from Impressive line, have been analyzed. DNAs, purified from leukocytes of those animals, were submitted to Polymerase Chain Reaction technique (PCR) and digested by Taq I restriction
enzyme. Accordingly the Hyperkalemic Periodic Paralysis (HYPP) genomic diagnosis was established. The tests revealed that 9 animals were carrier heterozygotes (N/H) and 3 normal homozygotes (N/N). Since the development of PCR methodology, it has been possible to diagnose the problem and plan ways to control the spreading of this defective gene in the population, through detection of carrier and normal animals.

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Published
1996-09-01
How to Cite
Garcia, J. F., Gurgel, A., Visintin, J. A., Lunge, V. R., Duarte, M., & Bertolli, J. (1996). Use of DNA markers for genomic diagnosis of domestic animals: 2. Detection of point mutation causing Hyperkalemic Periodic Paralysis (HYPP) in Quarter horses. Brazilian Journal of Veterinary Research and Animal Science, 33(3), 136-139. https://doi.org/10.11606/issn.2318-3659.v33i3p136-139
Section
BASIC SCIENCES