Effect of glutathione on the function and oxidative status of ovine cryopreserved sperm

  • Eduardo Gualtieri de Andrade Perez Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Reprodução Animal, São Paulo, SP
  • Marcilio Nichi Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Reprodução Animal, São Paulo, SP
  • Carlos Henrique Cabral Viana Pontiícia Universidade Católica de Poços de Caldas, Faculdade de Medicina Veterinária, Poços de Caldas, MG
  • Rodrigo Otávio Correia da Silva Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Reprodução Animal, São Paulo, SP
  • Andressa Dalmazzo Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Reprodução Animal, São Paulo, SP
  • Paola Almeida de Araújo Góes Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Reprodução Animal, São Paulo, SP
  • João Rafael Chinait Gurgel Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Reprodução Animal, São Paulo, SP
  • Valquiria Hyppolito Barnabe Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Reprodução Animal, São Paulo, SP
  • Renato Campanarut Barnabe Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Reprodução Animal, São Paulo, SP

Abstract

The high susceptibility of sperm to the oxidative stress occurs especially due to high content of poly-unsaturated fattyacids (PUFAs) in its plasma membrane. The PUFAs provide the necessary fluidity to the plasma membrane. Howeverdouble bonds present in those fatty acids are more susceptible to oxidative stress. Studies in human indicate thatcryopreservation may lead to damages to the sperm due to oxidative stress. This study aimed to verify if the antioxidantglutathione (GSH) may protect ovine cryopreserved sperm against damages caused by oxidative stress. Semen samplesof four rams were cryopreserved using Tris-egg yolk extender supplemented with different concentrations of reducedglutathione (control, 1, 5 and 10 mM). After thawing, samples were evaluated using conventional (motility and vigor)and functional tests (membrane integrity and mitochondrial activity). Aliquots of each thawed sample were submitted toprotocol of induced lipid peroxidation using ascorbate (20 mM) and ferrous sulphate (4 mM), with further measurementof tiobarbituric acid reactive substances (TBARS), index of oxidative stress. No effect of GSH was observed on variablesassessed by conventional tests. GSH decreased the proportion of intact acrosomes. Samples treated with 5 mM GSHshowed lower percentage of intact membrane cells when compared to control samples and those treated with 10 mM.The percentage of cells with mitochondrial activity was affected by GSH, but no effect on TBARS. Samples from controlgroup were more susceptible to denaturation of chromatin. In conclusion, the addition of Glutathione (GSH) offersprotection to DNA and mitochondrial activity of ovine sperm.

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Published
2012-08-22
How to Cite
Perez, E., Nichi, M., Viana, C. H., Silva, R. O., Dalmazzo, A., Góes, P., Gurgel, J. R., Barnabe, V., & Barnabe, R. (2012). Effect of glutathione on the function and oxidative status of ovine cryopreserved sperm. Brazilian Journal of Veterinary Research and Animal Science, 49(4), 262-268. https://doi.org/10.11606/issn.1678-4456.v49i4p262-268
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