The influence of serum concentration on the sensitivity and specificity of the microscopic aglutination test, using the L. biflexa, Buenos Aires strain, as antigen for diagnosing swine leptospirosis

  • Silvio Arruda Vasconcelos Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, São Paulo, SP
  • Ioshimiro Ohtsubo Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, São Paulo, SP
  • Paulo Hideki Yasuda Universidade de São Paulo, Instituto de Ciências Biomédicas, São Paulo, SP
  • Anibal Sant'anna Moretti Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, São Paulo, SP
  • Fumio Honma Ito Instituto Biológico de São Paulo, São Paulo, SP
  • Estevão de Camargo Passos Instituto Biológico de São Paulo, São Paulo, SP
  • José de Angelis Côrtes Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, São Paulo, SP
Keywords: Leptospirosis in swine, Leptospira interrogans Leptospira biflexa, Soroagglutination

Abstract

The influence of serum concentration on the results of the microscopic agglutination test (MAT), using the L. biflexa strain Buenos Aires as antigen, was investigated in three experimental groups of swines.
The group "A" composed of nine animals was the control. The group "B" composed of ten animals had its water supply contaminated with the L. biflexa strain during the first three experimental days. The group "C" composed of ten animals was inoculated with a virulent strain of L. interrogans serovar pomona by the intraperitonial route. The blood collections were obtained on the experimental days 5th , 15th , 30th ,
39th and 51st . The serum dilutions tested were: 1:10; 1:20; 1:40; 1:80 and 1:100. The amounts of negative results in group "A" represented the specificity of the test and the amounts of positive results in group
"C" the sensitivity. The results showed that when the concentration of the serum increases there was also an increase in its sensitivity but there was a decrease in its specificity. The best results of both of these characteristics were 60% at the serum dilution of 1 :20

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Published
1990-06-01
How to Cite
Vasconcelos, S., Ohtsubo, I., Yasuda, P., Moretti, A., Ito, F., Passos, E. de, & Côrtes, J. de. (1990). The influence of serum concentration on the sensitivity and specificity of the microscopic aglutination test, using the L. biflexa, Buenos Aires strain, as antigen for diagnosing swine leptospirosis. Brazilian Journal of Veterinary Research and Animal Science, 27(1), 33-39. https://doi.org/10.11606/issn.0000-0000.27133-39
Section
PREVENTIVE VETERINARY MEDICINE

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