Detection of rabies virus antigen by direct immunoperoxidase and fluorescent antibody test in experimentally inoculated mice, sacrificed in assymptomatic and agonizing state.

  • Jane Megid Universidade Estadual de Londrina, Londrina, PR
  • Fumio Honma Ito Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, São Paulo, SP
Keywords: Rabies diagnosis, Fluorescent antibody technique, Imunological reactions, intnunoperoxidase, Mice

Abstract

The positivity of the direct immunoperoxidase and the fluorescent antibody test for rabies virus antigen was comparatively evaluated in sixty young mice inoculated intracerebrally with street rabies virus. Thirty mice were sacrificed in assymptomatic state and the others in their agonizing state. The positive results obtained from impression brain smears of assymptomatic animals by direct immunoperoxidase and fluorescent antibody test were 83.3% and 86.6%, respectively. The results found in impression brain smears of agonizing animals revelead 100% positivity in both techniques. The direct immunoperoxidase test presented a relative sensitivity comparable to the
fluorescent antibody test for the detection of rabies virus antigen.

Downloads

Download data is not yet available.
Published
1990-12-02
How to Cite
Megid, J., & Ito, F. (1990). Detection of rabies virus antigen by direct immunoperoxidase and fluorescent antibody test in experimentally inoculated mice, sacrificed in assymptomatic and agonizing state. Brazilian Journal of Veterinary Research and Animal Science, 27(2), 187-192. https://doi.org/10.11606/issn.0000-0000.272187-192
Section
PREVENTIVE VETERINARY MEDICINE

Most read articles by the same author(s)

1 2 > >>