Interaction of bovine alpha2 macroglobulin with proteinases

Authors

  • Maria Regina Torqueti Toloi Universidade de São Paulo, Faculdade de Ciências Farmacêuticas, Ribeirão Preto, SP.

DOI:

https://doi.org/10.11606/issn.1678-4456.bjvras.1994.52053

Keywords:

Cattle, Plasma, Proteinase inhibitors, Alpha2M, Plasmin, Trypsin

Abstract

The alpha2-macroglobulin (alpha2M) protease inhibitor was purified from bovine plasma. The alpha2M preparations at various purification steps were identified by immunodiffusion and crossed immunoelectrophoresis with anti-human alpha2 serum. Anti-bovine alpha2M serum was prepared for the quantitative determinations. The purest alpha2M preparation was obtained by affinity chromatography and used as primary standard in radial immunodiffusion. Alpha2M preparations were submitted to binding tests with p' - NPGB (p‘ - nitrophenyl-p-guanidinebenzoate HCL) titrated trypsin and plasmin. Alpha2M protected 35% of the esterolytic activity of trypsin and 50% of the amidolytic activity of plasmin.

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Published

1994-06-02

Issue

Vol. 31 No. 2 (1994)

Section

BASIC SCIENCES

License

The journal content is authorized under the Creative Commons  BY-NC-SA  license (summary of the license: https://creativecommons.org/licenses/by-nc-sa/4.0 | full text of the license: https://creativecommons.org/licenses/by-nc-sa/4.0/legalcode) 

How to Cite

1.
Toloi MRT. Interaction of bovine alpha2 macroglobulin with proteinases. Braz. J. Vet. Res. Anim. Sci. [Internet]. 1994 Jun. 2 [cited 2024 Apr. 26];31(2):101-5. Available from: https://revistas.usp.br/bjvras/article/view/52053