Use of dried blood sample for serological diagnosis of toxoplasmosis in goats

  • Maria Terezinha Bahia Universidade de Alfenas, MG.
  • Ricardo Wagner de Almeida Vitor Universidade Federal de Minas Gerais, MG.
  • George Luiz Lins Machado Coelho Universidade Federal de Ouro Preto, MG.
  • Rogerio Pinheiro Caldas CAPRILEITE, Belo Horizonte, MG.
  • Carlos Maurício Figueiredo Antunes Universidade Federal de Minas Gerais, MG.
  • Cléa de Andrade Chiari Universidade Federal de Minas Gerais, MG.
Keywords: Blood specimen collection, Toxoplasmosis, Goats

Abstract

Eluates of 183 dried caprine blood samples on filter paper were tested for anti-Toxoplasma gondii antibodies, using IFA, ELISA and dot-ELISA, and compared with results obtained by direct observations on comparable sera, usin the same tests. The results has showed that papers with dried blood can be preserved for up to 45 days at room temperature and for six months at 4°C, provided they are protected against humid conditions by using desiccating agents such as silica-gel. Comparison between serum and eluate reactions revelated 97-100% correspondance. The antibody titers remained constant throughout the study period.

Downloads

Download data is not yet available.
Published
1995-06-02
How to Cite
Bahia, M. T., Vitor, R. W., Coelho, G. L., Caldas, R., Antunes, C. M., & Chiari, C. (1995). Use of dried blood sample for serological diagnosis of toxoplasmosis in goats. Brazilian Journal of Veterinary Research and Animal Science, 32(2), 83-88. https://doi.org/10.11606/issn.1678-4456.bjvras.1994.52092
Section
PREVENTIVE VETERINARY MEDICINE