Proteomics to investigate bull’s semen freezability

  • Marcelo Roncoletta Fazenda Lagoinha, Top in Life Biotecnologia & Genética Animal, Jaboticabal, SP
  • Erika da Silva Carvalho Morani Fazenda Lagoinha, Top in Life Biotecnologia & Genética Animal, Jaboticabal, SP
  • Lúcia Helena Rodrigues
  • Paulo Henrique Francheschini Universidade Estadual Paulista, Faculdade de Ciências Agrárias e Veterinárias, Departamento de Reprodução, Jaboticabal, SP
Keywords: Cattle, Proteins, Seminal Plasma, Cryopreservation

Abstract

The present study was undertaken the protein composition in 2D-electrophoretic pattern (2DE) of the seminal plasma (SP) can interfere in the semen bull freezability, and if we can use that for predicting semen bull freezability. Samples were obtained of 20 bulls (different breeds) with a minimum of 3 years history semen production in commercial semen collection Center. All animals ranged between 2 - 7 years of age. The semen freezability was calculated by # of thawed and approved ejaculates / # of ejaculates submitted to cryopreservation (after semen evaluation and approved to submitted to freeze). The bulls were divided in 3 groups: HIGH (=>80% ejaculates approved); MEDIUM (>60% and <79% ejaculates approved); LOW (=<59% ejaculates approved); the pattern and criteria were the same used in the routine of the commercial semen Center. 68 gels were carried through by 2DE of SP samples indicated 225 detected spots with protein different amount (VION) comparing. Comparing bull´s semen freezability and VION of each spot found difference among 2 spots from High and Low, even considering just spots with % of detection frequency bigger than 75%. The taurine bulls demonstrated more homogeneous profile when comparing with zebu bulls, considering number and frequency of appearance of spots. The results showed that proteomics can be a useful tool to predict the semen freezability, but we´ll need to study better the interactions between sperm membrane, seminal plasma and extender to comprehend better which proteome phenotype interfere positive or negatively in the semen freezability.

Downloads

Download data is not yet available.
Published
2013-02-22
How to Cite
Roncoletta, M., Morani, E. da, Rodrigues, L. H., & Francheschini, P. H. (2013). Proteomics to investigate bull’s semen freezability. Brazilian Journal of Veterinary Research and Animal Science, 50(1), 43-49. https://doi.org/10.11606/issn.2318-3659.v50i1p43-49
Section
UNDEFINIED