Morphometric evaluation of the epididymal area and efferent ducts and epididymal ducts in the domestic quail, throughout the year

Abstract

A morphometrical study of the cranial, medial and caudal portions of the epididymis from 72 domestic quail was performed during one year, being six epididymides collected each month. The epididymal portions were morphometrically evaluated through five histological slides (7 µm thick) from each bird. The slides were stained by Harris hematoxilin. The morphometrical measures were taken from 4 transversal sections, randomly chosen, from each organs (120 measurements per bird each month). Measures were taken from the epididymal area. The diameter and thickness of the epithelium in the proximal and distal efferent ductules and the epididymal ducts were also measured, using 40x magnification lenses and an image analyzer coupled with a binocular Zeiss microscope. Statistical analyses were performed using the SAS computer program. The analyses of the results showed that the caudal portion of the epididymus was significantly larger than the cranial portion when comparing the epididymal area and the major diameter of the distal and epididymal efferent ductules. These data suggested that the caudal portion is more active than the medial and cranial ones, similar to what occurs in mammals.