Diagnostic efficiency of Brucella soluble antigens in immunodiffusion tests and ability to differentiate Brucella abortus strain 19 vaccinated cattle

Authors

  • José Daffner University of Chile, Faculty of Veterinary and Animal Sciences, Department of Preventive Veterinary Medicine, Infectious Diseases Laboratory, Santiago
  • Pedro Abalos University of Chile, Faculty of Veterinary and Animal Sciences, Department of Preventive Veterinary Medicine, Infectious Diseases Laboratory, Santiago
  • Lautaro Pinochet University of Chile, Faculty of Veterinary and Animal Sciences, Department of Preventive Veterinary Medicine, Infectious Diseases Laboratory, Santiago
  • Mariela Scortti University of Chile, Faculty of Veterinary and Animal Sciences, Department of Preventive Veterinary Medicine, Infectious Diseases Laboratory, Santiago
  • Santiago Urcelay University of Chile, Faculty of Veterinary and Animal Sciences, Department of Preventive Veterinary Medicine, Infectious Diseases Laboratory, Santiago

DOI:

https://doi.org/10.1590/S1413-95961999000300002

Keywords:

rucellosis, Immunodiffusion tests, Vaccination, Cattle

Abstract

Three soluble antigens were compared by radial immunodiffusion (RID) and agar gel immunodiffusion (AGID) tests: a native haptene (NH) from Brucella melitensis 16M, and a polysaccharide (PS) from B. abortus 1119-3, both obtained by non-hydrolytic methods, and the (O-Chain) polysaccharide extracted also from B. abortus 1119-3 but using an hydrolytic method. Three groups of bovine sera were tested: a) Naturally infected (n = 76); b) Non-infected (n = 130) and c) S-19 vaccinated (n = 61); the sensitivity (Se), the specificity (Sp) and the ability to differentiate vaccinated (ADV) were determined in each group a, b and c respectively. The highest Se in the RID test (84.3%) was achieved by NH; while the three antigens gave 100% Sp. The O-Chain showed 100% ADV in this test. In the AGID test PS antigen showed the best Se (86.6%), and all antigens showed 100% of Sp and ADV. Finally, for its production qualities and efficiency the antigens PS and NH represent a promising alternative for complementary diagnosis of brucellosis.

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Published

1999-01-01

Issue

Section

VETERINARY MEDICINE

How to Cite

Diagnostic efficiency of Brucella soluble antigens in immunodiffusion tests and ability to differentiate Brucella abortus strain 19 vaccinated cattle. (1999). Brazilian Journal of Veterinary Research and Animal Science, 36(3), 116-120. https://doi.org/10.1590/S1413-95961999000300002