Primary culture of hepatic cells from Metynnis roosevelti (Pisces, Teleostei, Characidae)

  • Ligia Maria Salvo Universidade Federal do Paraná, Centro de Diagnóstico Marcos Enrietti, Curitiba, PR
  • Maria Ivette Carboni Malucelli Pontifícia Universidade Católica do Paraná, Centro de Ciências Biológicas e da Saúde, Curitiba, PR
  • Rosária Regina Tesoni B. Richartz Universidade Federal do Paraná, Centro de Diagnóstico Marcos Enrietti, Curitiba, PR
  • Metry Bacila Pontifícia Universidade Católica do Paraná, Centro de Ciências Biológicas e da Saúde, Curitiba, PR
Keywords: Liver cells, Cell culture, Cells, Pisces, Metynnis roosevelti

Abstract

In order to achieve monolayers and adhesion in cell cultures of hepatocytes from the subtropical fish Metynnis roosevelti (Pisces, Teleostei, Characidae) three different methodologies were used: enzyme cell dissociation by means of a system containing trypsin and versene 0.25%; by means of bovine fetal serum and by the use of blood serum obtained from adult specimens of M. roosevelti. The cell medium used has been a modified F10-199 by the addition of 2 mM L-glutamine, 10 µg/mL insulin, 50 µg/mL fibronectin; and antibiotics. The best results in terms of cell dissociation as well as in terms of adhesion and confluence of the cells monolayer has been achieved by means of the enzymatic cell dissociation.

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Published
2000-01-01
How to Cite
Salvo, L. M., Malucelli, M. I., Richartz, R. R., & Bacila, M. (2000). Primary culture of hepatic cells from Metynnis roosevelti (Pisces, Teleostei, Characidae). Brazilian Journal of Veterinary Research and Animal Science, 37(5), 366-371. https://doi.org/10.1590/S1413-95962000000500004
Section
BASIC SCIENCES

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