Inbred F344 rats as a biologic model of intra-abdominal sepsis

  • Sueli Blanes Damy Universidade de São Paulo, Faculdade de Medicina, Centro de Bioterismo, São Paulo, SP
  • Luci Ebisui Universidade de São Paulo, Faculdade de Medicina, Centro de Bioterismo, São Paulo, SP
  • Marilda Osti Spinelli Universidade de São Paulo, Faculdade de Medicina, Centro de Bioterismo, São Paulo, SP
  • Junko Takano Osaka Universidade de São Paulo, Faculdade de Medicina, Centro de Bioterismo, São Paulo, SP
  • Erasmo Magalhães Castro de Tolosa Universidade de São Paulo, Faculdade de Medicina, Centro de Bioterismo, São Paulo, SP
  • Silvia Colleta Barreto da Costa Ortiz Universidade de São Paulo, Faculdade de Medicina, Centro de Bioterismo, São Paulo, SP
Keywords: SEPSIS, Rats, Models (Biological)

Abstract

Aiming to design a biologic model of acute intra-abdominal sepsis for experimental studies, conventional, isogenic rats F344 were infected with the bacteria Escherichia coli (E.coli), strain 11775, serotype H7:01:K1. Six hours after inoculation with E. coli, the animals - males and females - showed the following symptoms: piloerection, hyperpnea and decreased motor activity. The lethal dosis (LD50), was 6 x 10(5) CFU/ml, analyzed in 32 males and 32 females. The highest mortality rate was observed on the first 24 hours. Liver dysfunction, common in intra-abdominal sepsis, was evaluated at 0, 24, 48 and 168 hours after inoculation, by means of serum enzyme activities. Study of the migration of polymorphonuclear-neutrophil cells (PMN) and mononuclear-macrophage ones (MN) showed a significant increase of PMN in E. coli inoculated males (z >; 4.7; P; 6.2; P < 0.0003), when compared to control groups. As for MN cells, there were no differences between inoculated and control groups, in males (z = 2.3; P = 0.0107) and in females (z = 1.8; P = 0.0359) as well. In conclusion, these results show that inbred F344 rats are adequate biologic models for studies of acute, intra-abdominal sepsis.

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Published
2002-01-01
How to Cite
Damy, S., Ebisui, L., Spinelli, M., Osaka, J., Tolosa, E., & Ortiz, S. (2002). Inbred F344 rats as a biologic model of intra-abdominal sepsis. Brazilian Journal of Veterinary Research and Animal Science, 39(1), 21-26. https://doi.org/10.1590/S1413-95962002000100004
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