Chemical carcinogenesis by DMBA (7,12-dimethylbenzanthracene) in female BALB/c mice: new facts

  • Krishna Duro de Oliveira Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Patologia
  • Gabriela Uliana Avanzo Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Patologia
  • Marcello Vannucci Tedardi Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Patologia
  • Marcelo Monte Mór Rangel Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Patologia
  • José Luis Avanzo Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Patologia
  • Heidge Fukumasu Universidade de São Paulo, Faculdade de Zootecnia e Engenharia de Alimentos
  • Kurapati Venkata Kesava Rao Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Patologia
  • Idércio Luiz Sinhorini Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Patologia
  • Maria Lúcia Zaidan Dagli Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Patologia
Keywords: Carcinogenesis, DMBA, Mice, Lung neoplasms, Breast neoplasms

Abstract

Polycyclic aromatic hydrocarbons are known carcinogens used in rodent experimental models. In this study, the carcinogen DMBA (7,12-dimethylbenzanthracene) was administered by gavage, diluted in corn oil, to female BALB / c mice at hebdomadary doses of 1 mg per animal for 1, 3, 6 or 9 weeks. Animals were weighed and monitored weekly until death. Remaining animals were euthanized at the age of 53 weeks. At necropsy, representative fragments of neoplasms were collected and routinely processed for histopathological analysis. Of all mice that received DMBA, 68.57% developed some type of tumor. Of the 70 mice treated with various doses of DMBA, 22 (31.43%) developed mammary tumors. The adenoacanthoma was the most commonly (18.75%) diagnosed histological type of breast cancer. Lung (15.71%), lymphoid tissue (11.43%), stomach (7.14%) and skin (2.86%) were also primary sites of tumor development. One third (33.33%) of the mice receiving 1 mg of DMBA developed lung cancer. Therefore, the administration of DMBA was shown to be an efficient model of carcinogenesis in mice, especially for the study of breast cancer, when using the highest dose, and lung, when using the lowest dose. Carcinogenesis models have been used for several purposes in cancer research. These results represent new facts for a classic carcinogenesis model.

Downloads

Download data is not yet available.
Published
2015-06-30
How to Cite
Oliveira, K., Avanzo, G., Tedardi, M., Rangel, M., Avanzo, J. L., Fukumasu, H., Rao, K., Sinhorini, I. L., & Dagli, M. L. (2015). Chemical carcinogenesis by DMBA (7,12-dimethylbenzanthracene) in female BALB/c mice: new facts. Brazilian Journal of Veterinary Research and Animal Science, 52(2), 125-133. https://doi.org/10.11606/issn.1678-4456.v52i2p125-133
Section
ARTICLES